Department of Laboratory Medicine, Myongji Hospital, Kwandong University College of Medicine, Goyang, South Korea.
Diagn Microbiol Infect Dis. 2013 Mar;75(3):308-12. doi: 10.1016/j.diagmicrobio.2012.11.025. Epub 2013 Jan 18.
In this study, we determined the prevalence of mupirocin and antiseptics resistance in methicillin-resistant Staphylococcus aureus (MRSA) at a secondary- and a tertiary-care hospital in Korea. Mupirocin susceptibility test, ileS gene sequencing, and polymerase chain reaction for mupA, qacA/B, and smr gene were performed with 456 nonduplicated MRSA isolates collected from 2 hospitals in Korea. Genetic relatedness was determined by spa typing and multilocus sequence typing (MLST). The rates of low-level and high-level mupirocin resistance were 7% and 2% in the secondary-care hospital and 17% and 2% in the tertiary-care hospital, respectively. The positive rate of qacA/B and smr gene in mupirocin-resistant MRSA was 65% and 71%, respectively. In spa typing, most mupirocin-resistant MRSA showed genetic relatedness and some of the highly resistant isolates were ST239 and ST5 in MLST analysis. The distribution of low-level and high-level mupirocin resistance and the coexistence of antiseptic resistance in MRSA can result in failure of decolonization in Korea.
在这项研究中，我们确定了韩国一家二级和一家三级医院耐甲氧西林金黄色葡萄球菌（MRSA）中莫匹罗星和防腐剂耐药的流行情况。我们对从韩国的 2 家医院收集的 456 株非重复的 MRSA 分离株进行了莫匹罗星药敏试验、ileS 基因测序以及 mupA、qacA/B 和 smr 基因的聚合酶链反应。通过 spa 分型和多位点序列分型（MLST）确定遗传相关性。二级医院中低水平和高水平莫匹罗星耐药率分别为 7%和 2%，三级医院中分别为 17%和 2%。莫匹罗星耐药 MRSA 中 qacA/B 和 smr 基因的阳性率分别为 65%和 71%。在 spa 分型中，大多数莫匹罗星耐药 MRSA 显示出遗传相关性，一些高度耐药的分离株在 MLST 分析中为 ST239 和 ST5。韩国 MRSA 中低水平和高水平莫匹罗星耐药以及防腐剂耐药共存的分布可能导致去定植失败。
