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短小芽孢杆菌 MTCC 12105:一种有潜力用于处理纸浆和造纸废水的细菌。

Brevibacillus parabrevis MTCC 12105: a potential bacterium for pulp and paper effluent degradation.

机构信息

Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonepat, Haryana, India.

Avantha Centre for Industrial Research and Development, Yamuna Nagar, Haryana, India.

出版信息

World J Microbiol Biotechnol. 2018 Jan 29;34(2):31. doi: 10.1007/s11274-018-2414-y.

Abstract

A gram positive, rod shaped, bacterium was isolated from pulp and paper mill sludge and characterized as Brevibacillus parabrevis (MTCC 12105) by biochemical tests and 16S rRNA gene sequencing. C and E stage wastewater, collected from a leading pulp and paper mill situated in North India, was used for degradation study. Wastewater degradation efficiency of the bacterial isolate was evaluated by Flask study in batch mode and Reactor study in semi continuous mode. The isolated bacterium showed a considerable reduction of the colour (59%), chemical oxygen demand (62%) and lignin (53.8%) content of Kraft paper mill effluent at 37 °C after 5 days. During reactor study it reduced 42.6% Lignin, 51.6% Colour and 60.3% COD (chemical oxygen demand) of the wastewater at 48 h RT (retention time). The isolate was deposited at MTCC Chandigarh, India with an accession no. MTCC 12105.

摘要

从纸浆和造纸厂污泥中分离出一种革兰氏阳性、杆状细菌,并通过生化试验和 16S rRNA 基因测序将其鉴定为 Brevibacillus parabrevis(MTCC 12105)。使用来自印度北部一家领先的纸浆和造纸厂的 C 和 E 阶段废水进行降解研究。通过分批摇瓶试验和半连续式反应器试验评估了细菌分离株对废水的降解效率。在 37°C 下,分离出的细菌在 5 天内使 kraft 纸浆厂废水中的颜色(59%)、化学需氧量(62%)和木质素(53.8%)含量显著降低。在反应器研究中,该菌在 48 小时 RT(保留时间)时将废水中的木质素、颜色和化学需氧量(COD)分别降低了 42.6%、51.6%和 60.3%。该分离株已在印度昌迪加尔的 MTCC 保藏, accession no. 为 MTCC 12105。

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