German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, Heidelberg 69120, Germany.
EMBO Rep. 2013 Mar 1;14(3):261-8. doi: 10.1038/embor.2013.1. Epub 2013 Jan 22.
The molecular mechanisms regulating tissue size represent an unsolved puzzle in developmental biology. One signalling pathway controlling growth of the Drosophila wing is Dpp. Dpp promotes growth by repression of the transcription factor Brk. The transcriptional targets of Brk that control cell growth and proliferation, however, are not yet fully elucidated. We report here a genome-wide ChIP-Seq of endogenous Brk from wing imaginal discs. We identify the growth regulator Myc as a target of Brk and show that repression of Myc and of the miRNA bantam explains a significant fraction of the growth inhibition caused by Brk. This work sheds light on the effector mechanisms by which Dpp signalling controls tissue growth.
调控组织大小的分子机制是发育生物学中的一个未解难题。在控制果蝇翅膀生长的信号通路中，Dpp 是其中之一。Dpp 通过抑制转录因子 Brk 来促进生长。然而，控制细胞生长和增殖的 Brk 的转录靶标尚未完全阐明。我们在此报告了来自翅膀 imaginal discs 的内源性 Brk 的全基因组 ChIP-Seq。我们鉴定出生长调节剂 Myc 是 Brk 的靶标，并表明 Myc 和 bantam miRNA 的抑制解释了 Brk 引起的生长抑制的很大一部分。这项工作揭示了 Dpp 信号控制组织生长的效应机制。
