Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO, USA.
Chem Commun (Camb). 2013 Feb 28;49(17):1759-61. doi: 10.1039/c3cc38871a.
We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage-DNA bioconjugates. DNA oligonucleotides were conjugated to M13 through acyl hydrazone linkages. In one case, DNAzymes retained their catalytic ability when anchored to the virus coat, and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild conditions.
我们报告了 M13 噬菌体-DNA 生物缀合物的合成、表征和蛋白质传感能力。通过酰腙键将 DNA 寡核苷酸与 M13 缀合。在一种情况下，当 DNA 酶附着在病毒外壳上时，它们保留了催化能力，而在另一项研究中，腙的动态性质允许在温和条件下从噬菌体中释放 DNA。
