Lobo Daniela P, Wemyss Alan M, Smith David J, Straube Anne, Betteridge Kai B, Salmon Andrew H J, Foster Rebecca R, Elhegni Hesham E, Satchell Simon C, Little Haydn A, Pacheco-Gómez Raúl, Simmons Mark J, Hicks Matthew R, Bates David O, Rodger Alison, Dafforn Timothy R, Arkill Kenton P
Department of Chemistry and Warwick Analytical Science Centre, University of Warwick, Coventry CV4 7AL, UK.
Department of Chemistry and Warwick Analytical Science Centre, University of Warwick, Coventry CV4 7AL, UK; MOAC Doctoral Training Centre, University of Warwick, Coventry CV4 7AL, UK.
Nano Res. 2015 Oct;8(10):3307-3315. doi: 10.1007/s12274-015-0831-x.
The wall shear stress (WSS) that a moving fluid exerts on a surface affects many processes including those relating to vascular function. WSS plays an important role in normal physiology (e.g. angiogenesis) and affects the microvasculature's primary function of molecular transport. Points of fluctuating WSS show abnormalities in a number of diseases; however, there is no established technique for measuring WSS directly in physiological systems. All current methods rely on estimates obtained from measured velocity gradients in bulk flow data. In this work, we report a nanosensor that can directly measure WSS in microfluidic chambers with sub-micron spatial resolution by using a specific type of virus, the bacteriophage M13, which has been fluorescently labeled and anchored to a surface. It is demonstrated that the nanosensor can be calibrated and adapted for biological tissue, revealing WSS in micro-domains of cells that cannot be calculated accurately from bulk flow measurements. This method lends itself to a platform applicable to many applications in biology and microfluidics.
运动流体作用于表面的壁面剪应力（WSS）会影响许多过程，包括与血管功能相关的过程。WSS在正常生理过程（如血管生成）中起重要作用，并影响微血管的分子运输主要功能。WSS波动点在多种疾病中表现出异常；然而，目前尚无在生理系统中直接测量WSS的既定技术。所有现有方法都依赖于从总体流量数据中测得的速度梯度获得的估计值。在这项工作中，我们报告了一种纳米传感器，该传感器可通过使用一种特定类型的病毒——荧光标记并锚定在表面的噬菌体M13，以亚微米空间分辨率直接测量微流控腔室中的WSS。结果表明，该纳米传感器可以校准并适用于生物组织，揭示细胞微区中的WSS，而这些WSS无法从总体流量测量中准确计算得出。这种方法适用于一个适用于生物学和微流控领域许多应用的平台。
