Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing, China.
Toxicol Mech Methods. 2013 Jul;23(6):383-8. doi: 10.3109/15376516.2013.769656. Epub 2013 Apr 23.
The aim of this study was to investigate the effects of vitamin C on cisplatin (DDP)-induced anemia and explore its possible mechanisms in rats. Adult male Sprague-Dawley rats were randomly divided into six groups: control, vitamin C 50, vitamin C 100, DDP, DDP plus vitamin C 50 and DDP plus vitamin C 100-treated groups. DDP was intravenous injected as a single dose and vitamin C was administered by gavage. Serum erythropoietin (Epo), hemoglobin (Hb) and blood urea nitrogen (BUN) concentration were measured 4 and 14 days after DDP treatment. The changes of renal tissue were examined by light microscope. Administration of DDP to rats induced anemia and nephrotoxicity, characterized with a significant decrease in serum Epo and Hb and increase in BUN concentrations. Pathological examination revealed that DDP caused significant renal damage in rats. Vitamin C administration produced amelioration in biochemical indices of anemia and nephrotoxicity and in histological change when compared to group DDP alone; concurrent administration of vitamin C at doses of 100 mg/kg being more effective. Results from this study indicate that the novel natural antioxidant vitamin C might have protective effect against DDP-induced anemia in rats.
本研究旨在探讨维生素 C 对顺铂（DDP）诱导性贫血的影响，并探索其在大鼠体内的可能机制。成年雄性 Sprague-Dawley 大鼠随机分为六组：对照组、维生素 C50 组、维生素 C100 组、DDP 组、DDP 联合维生素 C50 组和 DDP 联合维生素 C100 组。DDP 以单次静脉注射给药，维生素 C 经灌胃给药。DDP 处理后第 4 天和第 14 天测量血清促红细胞生成素（Epo）、血红蛋白（Hb）和血尿素氮（BUN）浓度。通过光镜检查观察肾组织的变化。DDP 给药导致大鼠贫血和肾毒性，表现为血清 Epo 和 Hb 显著降低，BUN 浓度升高。病理检查显示 DDP 导致大鼠肾脏严重损伤。与 DDP 组相比，维生素 C 给药可改善贫血和肾毒性的生化指标，并改善组织学变化；当维生素 C 以 100mg/kg 的剂量联合给药时，效果更为显著。本研究结果表明，新型天然抗氧化剂维生素 C 可能对大鼠的 DDP 诱导性贫血具有保护作用。
