Geyikoğlu Fatime, Çolak Suat, Türkez Hasan, Bakır Murat, Koç Kübra, Hosseinigouzdagani Mir Khalil, Çeriğ Salim, Sönmez Merve
Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey.
Üzümlü Vocational School, Erzincan University, Erzincan, Turkey.
Indian J Hematol Blood Transfus. 2017 Sep;33(3):348-354. doi: 10.1007/s12288-016-0718-3. Epub 2016 Aug 16.
The prevalence of cancer, in the world is increasing steadily. Despite intense research efforts, no approved therapy is yet available. Cisplatin is a chemotherapeutic drug but induces acute tissue injury. Oleuropein (OLE) is a major phenolic compound and used as a possible natural antioxidant, antimicrobial, and anticancer agent. We hypothesized that antioxidant activity of OLE may decrease cisplatin-induced oxidative stress and prevent to the development of chemotherapeutic complications including abnormality in hematological condition. Male Sprague Dawley rats were used in the experiments. Rats were randomly assigned to one of eight groups: control group; group treated with i.p. injection in a single dose of 7 mg/kg/day cisplatin; groups treated with 50, 100 and 200 mg/kg/day OLE (i.p.); and groups treated with OLE for 3 days starting at 24 h following cisplatin injection. First, hematological assessment was appreciated between control and experimental groups. Second, total oxidative stress (TOS) and total antioxidant capacity (TAC) levels of blood were measured by biochemical studies. In addition to this, oxidative DNA damage was determined by measuring as increases in 8-hydroxy-deoxyguanosine (8-OH-dG) adducts. The treatment with cisplatin elevated the TOS and 8-OH-dG levels that were then reversed by OLE. Reductions in antioxidant capacity with respect to corresponding controls were also restored by OLE treatment. These findings suggest that the OLE treatment against cisplatin-induced toxicity improves the function of blood cells and helps them to survive in the belligerent environment created by free radicals.
全球癌症患病率正在稳步上升。尽管进行了大量研究，但仍未出现获批的治疗方法。顺铂是一种化疗药物，但会引发急性组织损伤。橄榄苦苷（OLE）是一种主要的酚类化合物，可用作潜在的天然抗氧化剂、抗菌剂和抗癌剂。我们推测，OLE的抗氧化活性可能会降低顺铂诱导的氧化应激，并预防化疗并发症的发生，包括血液学状况异常。实验采用雄性Sprague Dawley大鼠。大鼠被随机分为八组之一：对照组；腹腔注射单剂量7mg/kg/天顺铂的组；腹腔注射50、100和200mg/kg/天OLE的组；以及在顺铂注射后24小时开始用OLE治疗3天的组。首先，对对照组和实验组进行血液学评估。其次，通过生化研究测量血液中的总氧化应激（TOS）和总抗氧化能力（TAC）水平。除此之外，通过测量8-羟基脱氧鸟苷（8-OH-dG）加合物的增加来确定氧化性DNA损伤。顺铂治疗使TOS和8-OH-dG水平升高，而OLE可使其逆转。OLE治疗也恢复了相对于相应对照组抗氧化能力的降低。这些发现表明，OLE对顺铂诱导的毒性的治疗改善了血细胞的功能，并帮助它们在自由基产生的恶劣环境中存活。
