Szegedi Krisztina, Göblös Anikó, Bacsa Sarolta, Antal Mária, Németh István Balázs, Bata-Csörgő Zsuzsanna, Kemény Lajos, Dobozy Attila, Széll Márta
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, H-6720 Szeged, Hungary.
Int J Mol Sci. 2012 Dec 21;14(1):205-25. doi: 10.3390/ijms14010205.
PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated variable expressions in different human tissues and a consistent staining pattern in epidermal keratinocytes and in vitro cultured keratinocytes. To identify the cellular function(s) of PRINS, we searched for a direct interacting partner(s) of this stress-induced molecule. In HaCaT and NHEK cell lysates, the protein proved to be nucleophosmin (NPM) protein as a potential physical interactor with PRINS. Immunohistochemical experiments revealed an elevated expression of NPM in the dividing cells of the basal layers of psoriatic involved skin samples as compared with healthy and psoriatic uninvolved samples. Others have previously shown that NPM is a ubiquitously expressed nucleolar phosphoprotein which shuttles to the nucleoplasm after UV-B irradiation in fibroblasts and cancer cells. We detected a similar translocation of NPM in UV-B-irradiated cultured keratinocytes. The gene-specific silencing of PRINS resulted in the retention of NPM in the nucleolus of UV-B-irradiated keratinocytes; suggesting that PRINS may play a role in the NPM-mediated cellular stress response in the skin.
PRINS是我们研究小组先前鉴定出的一种非编码RNA，它与银屑病易感性和细胞应激反应有关。我们现在通过原位杂交研究了PRINS的细胞和组织学分布，并证明其在不同人体组织中的表达存在差异，而在表皮角质形成细胞和体外培养的角质形成细胞中具有一致的染色模式。为了确定PRINS的细胞功能，我们寻找了这种应激诱导分子的直接相互作用伙伴。在HaCaT和NHEK细胞裂解物中，该蛋白被证明是核仁磷酸蛋白（NPM），它是与PRINS潜在的物理相互作用分子。免疫组织化学实验显示，与健康和未受累的银屑病皮肤样本相比，NPM在银屑病受累皮肤样本基底层的分裂细胞中表达升高。其他人先前已表明，NPM是一种普遍表达的核仁磷蛋白，在成纤维细胞和癌细胞中经紫外线B照射后会穿梭至核质。我们在紫外线B照射的培养角质形成细胞中检测到了类似的NPM易位。PRINS基因特异性沉默导致NPM保留在紫外线B照射的角质形成细胞的核仁中；这表明PRINS可能在皮肤中NPM介导的细胞应激反应中发挥作用。
