人淋巴细胞分泌免疫反应性生长激素（GH）：外源性GH增强其释放

Immunoreactive growth hormone (GH) secretion by human lymphocytes: augmented release by exogenous GH.

作者信息

Hattori N, Shimatsu A, Sugita M, Kumagai S, Imura H

机构信息

Department of Internal Medicine, Kyoto University Faculty of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Apr 30;168(2):396-401. doi: 10.1016/0006-291x(90)92334-v.

DOI:10.1016/0006-291x(90)92334-v

PMID:2334410

Abstract

Peripheral blood mononuclear cells (PBMCs) from normal adults secreted small amounts of human growth hormone (GH; 0.2-0.6 pg/10(5) cells/7 days culture) as measured by a highly sensitive enzyme immunoassay. Stimulation of PBMCs with phytohemagglutinin (PHA) consistently showed a 4-6 fold increase in GH secretion. Transformed B-lymphocytes by Epstein-Barr virus also secreted GH (0.8-4.8 pg/5 x 10(4) cells/7 days culture). GH secreted by lymphocytes comigrated with pituitary GH on an Ultrogel AcA44 column. Addition of GH during the culture augmented endogenous GH secretion from PHA-stimulated PBMCs. GH-releasing hormone and a somatostatin analogue, SMS 201-995, did not affect GH secretion from non-stimulated and PHA-stimulated PBMCs. These findings suggest that both T and B lymphocytes secrete immunoreactive GH in a different manner from that in the anterior pituitary.

摘要

通过高灵敏度酶免疫测定法检测发现，正常成年人的外周血单个核细胞（PBMC）分泌少量人生长激素（GH；0.2 - 0.6 pg/10⁵细胞/7天培养）。用植物血凝素（PHA）刺激PBMC后，GH分泌量持续增加4至6倍。经爱泼斯坦 - 巴尔病毒转化的B淋巴细胞也分泌GH（0.8 - 4.8 pg/5×10⁴细胞/7天培养）。淋巴细胞分泌的GH在Ultrogel AcA44柱上与垂体GH共迁移。培养期间添加GH可增强PHA刺激的PBMC的内源性GH分泌。生长激素释放激素和生长抑素类似物SMS 201 - 995对未刺激和PHA刺激的PBMC的GH分泌均无影响。这些发现表明，T淋巴细胞和B淋巴细胞均以不同于垂体前叶的方式分泌免疫反应性GH。