微板 alamarBlue 法用于检测泊拉卡氏菌的敏感性。
Microplate alamarBlue assay for Paracoccidioides susceptibility testing.
机构信息
Faculdade de Ciências Farmacêuticas, UNESP-Universidade Estadual Paulista, Araraquara, Departamento de Analises Clinicas, Laboratório de Micologia Clínica, São Paulo, Brazil.
出版信息
J Clin Microbiol. 2013 Apr;51(4):1250-2. doi: 10.1128/JCM.02914-12. Epub 2013 Jan 23.
Abstract
CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs.
摘要
CLSI 方法 M27-A3 不适用于二相性真菌，如那些属于荚膜组织胞浆菌属的真菌。在这项研究中，我们开发了一种微量稀释法，并添加了 alamarBlue 试剂来测试巴西副球孢子菌和卢氏副球孢子菌对两性霉素 B 和伊曲康唑抗真菌药物的反应。该试验证明是敏感、实用和廉价的，可以用于监测低生长微生物的活性及其对各种药物的反应。
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