Ishii Y, Ishijima A, Yanagida T
Single Molecule Processes Project, ICORP, JST, 2-4-14 Senba-higashi Mino, 562-0035, Osaka, Japan.
Trends Biotechnol. 2001 Jun;19(6):211-6. doi: 10.1016/s0167-7799(01)01635-3.
The development of nanomanipulation techniques has given investigators the ability to manipulate single biomolecules and to record mechanical events of biomolecules at the single molecule level. The techniques were developed to elucidate the mechanism of molecular motors. We can directly monitor the unitary process of the mechanical work and the energy conversion processes by combining these techniques with the single molecule imaging techniques. Our results strongly suggest that the sliding movement of the actomyosin motor is driven by Brownian movement. Other groups have reported data that are more consistent with the lever arm model. These methods and imaging techniques enable us to monitor the behavior of biomolecules at work and will be applied to other molecular machines.
纳米操纵技术的发展使研究人员能够操纵单个生物分子，并在单分子水平上记录生物分子的机械事件。这些技术是为阐明分子马达的机制而开发的。通过将这些技术与单分子成像技术相结合，我们可以直接监测机械功的单一过程和能量转换过程。我们的结果有力地表明，肌动球蛋白马达的滑动运动是由布朗运动驱动的。其他研究小组报告的数据与杠杆臂模型更一致。这些方法和成像技术使我们能够监测工作中的生物分子的行为，并将应用于其他分子机器。
