Ikai A, Idiris A, Wang T, Alam M T, Afrin R, Hyonchol K, Sekiguchi H, Nishida S, Arakawa H, Osada T
Laboratory of Biodynamics, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta Midoriku, Yokohama, 226-8501 Japan.
J Biol Phys. 2002 Dec;28(4):561-72. doi: 10.1023/A:1021252017832.
The atomic force microscope is currently used in our and many other laboratories to measure the mechanical response of polypeptide and proteins against tensile forces applied to well defined positions in their chemical structures. The resulting force vs. extension (F-E) curves are analyzed in relation to their known conformations under various conditions. The method can be extended to study the mechanical responses of other, often much larger biological structures, and extract the component proteins and DNAs from cell membranes and chromosomes.
目前，我们实验室和许多其他实验室都在使用原子力显微镜来测量多肽和蛋白质在其化学结构中特定位置所受拉力时的力学响应。根据所得的力与伸长（F-E）曲线，分析它们在各种条件下的已知构象。该方法可扩展用于研究其他通常大得多的生物结构的力学响应，并从细胞膜和染色体中提取组成蛋白和DNA。
