Takaba Katsumi, Imada Teruyoshi, Katsumata Shigehisa, Okumura Hiroshi, Iwamoto Sachiko, Suzuki Yui, Imaizumi Minami, Myojo Kensuke, Takada Chie, Kimoto Naoya, Saeki Koji, Yamaguchi Itaru
Toxicological Research Laboratories, Kyowa Hakko Kirin Co., Ltd., 1188 Shimotogari, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8731, Japan.
J Toxicol Pathol. 2012 Dec;25(4):265-71. doi: 10.1293/tox.25.265. Epub 2012 Dec 20.
This study histopathologically and immunohistochemically investigated a spontaneously occurring single mass subcutaneously located in the left lower abdomen of a female BALB/cAJcl-nu/+ mouse at 10 weeks of age. The mass was about 20 × 15 × 10 mm in size after formalin fixation; nevertheless, it was not detected by clinical observations at 9 weeks of age. H&E staining revealed the tumor origin was epithelial and probably arose from the mammary gland, and the tumor cells demonstrated a squamous, acinar or polyhedral/basal pattern. A cell kinetics analysis revealed that many of the tumor cells of the squamous, acinar or polyhedral/basal component were positive for PCNA and cyclin D1, although there were a few of TUNEL-positive tumor cells in all of the components. An epithelial/mesenchymal analysis demonstrated that most of the tumor cells of the squamous and acinar components contained keratin and E-cadherin; however, most of the tumor cells of the polyhedral/basal component were less or very weakly positive for these markers. The tumor cells of the squamous component were negative for vimentin and SMA; however, many of the tumor cells of the polyhedral/basal component exhibited vimentin. In addition, expression of SMA was confirmed in some tumor cells of the acinar and basal components. Based on the microscopic and immunohistochemical characterizations, the tumor was diagnosed to be adenosquamous carcinoma that originated from the mammary gland with rapid growth, and the tumor cells demonstrated epithelial-mesenchymal transition-like changes.
本研究采用组织病理学和免疫组织化学方法，对一只10周龄雌性BALB/cAJcl-nu/+小鼠左下腹自发形成的皮下单个肿块进行了研究。经福尔马林固定后，肿块大小约为20×15×10mm；然而，在9周龄时临床观察未发现该肿块。苏木精-伊红染色显示肿瘤起源于上皮组织，可能来自乳腺，肿瘤细胞呈鳞状、腺泡状或多面体/基底样形态。细胞动力学分析显示，鳞状、腺泡状或多面体/基底样成分的许多肿瘤细胞PCNA和细胞周期蛋白D1呈阳性，尽管所有成分中都有少数TUNEL阳性肿瘤细胞。上皮/间充质分析表明，鳞状和腺泡状成分的大多数肿瘤细胞含有角蛋白和E-钙黏蛋白；然而，多面体/基底样成分的大多数肿瘤细胞对这些标志物呈弱阳性或极弱阳性。鳞状成分的肿瘤细胞波形蛋白和平滑肌肌动蛋白呈阴性；然而，多面体/基底样成分的许多肿瘤细胞波形蛋白呈阳性。此外，在腺泡状和基底样成分的一些肿瘤细胞中证实有平滑肌肌动蛋白表达。根据显微镜和免疫组织化学特征，该肿瘤被诊断为起源于乳腺的腺鳞癌，生长迅速，肿瘤细胞表现出上皮-间充质转化样改变。
