Driscoll P G, Joseph F, Nakamoto T
Department of Physiology, Louisiana State University Medical Center, New Orleans 70119.
Br J Nutr. 1990 Mar;63(2):285-92. doi: 10.1079/bjn19900115.
The purpose of the present study was to determine the effects of caffeine on the mandibles of newborn rats whose dams were given a normal diet (200 g protein/kg diet) compared with those given a high-protein diet (400 g protein/kg diet) during gestation. A total of twenty pregnant Sprague-Dawley rats were randomly divided into four groups of five each. Starting on day 7 of gestation, groups 1 and 2 were fed on control and high-protein diets respectively, and groups 3 and 4 were pair-fed with groups 1 and 2 respectively, but with caffeine added to their diets. The caffeine supplement was 20 mg/kg body-weight. At birth, pups were killed and various measurements of their mandibles were made. The mandibular weights, calcium contents, and alkaline (EC 3.1.3.1) and acid (EC 3.1.3.2) phosphatase activities of the group given the caffeine-supplemented control diet were significantly lower than those of the corresponding unsupplemented group. Alkaline and acid phosphatase activities, collagen synthesis and hydroxyproline contents of the caffeine-supplemented high-protein group were greater than those of the corresponding unsupplemented group, whereas Ca and protein contents of the caffeine-supplemented high-protein group were lower than those of the corresponding unsupplemented group. There were no significant differences in plasma caffeine levels for either dams or pups between the caffeine-supplemented control and high-protein groups. The effects of caffeine on the development of fetal mandibles are apparently modified by different levels of maternal dietary protein.
本研究的目的是确定咖啡因对新生大鼠下颌骨的影响，这些新生大鼠的母鼠在妊娠期分别给予正常饮食（200克蛋白质/千克饮食）和高蛋白饮食（400克蛋白质/千克饮食）。总共20只怀孕的斯普拉格-道利大鼠被随机分为四组，每组五只。从妊娠第7天开始，第1组和第2组分别给予对照饮食和高蛋白饮食，第3组和第4组分别与第1组和第2组配对喂养，但在饮食中添加咖啡因。咖啡因补充量为20毫克/千克体重。出生时，将幼崽处死并对其下颌骨进行各种测量。给予补充咖啡因的对照饮食组的下颌骨重量、钙含量以及碱性（EC 3.1.3.1）和酸性（EC 3.1.3.2）磷酸酶活性显著低于相应的未补充组。补充咖啡因的高蛋白组的碱性和酸性磷酸酶活性、胶原蛋白合成和羟脯氨酸含量高于相应的未补充组，而补充咖啡因的高蛋白组的钙和蛋白质含量低于相应的未补充组。补充咖啡因的对照饮食组和高蛋白组的母鼠或幼崽的血浆咖啡因水平没有显著差异。咖啡因对胎儿下颌骨发育的影响显然受到母体饮食蛋白质不同水平的影响。
