Sasahara H, Yamano H, Nakamoto T
Department of Oral Diagnosis, Nihon University School of Dentistry, Matsudo, Japan.
Arch Oral Biol. 1990;35(6):425-30. doi: 10.1016/0003-9969(90)90204-n.
On day 9 of gestation, pregnant dams were randomly divided into 3 groups. Dams of group 1 were fed a 20% protein diet as a control. Dams of group 2 were fed a 20% protein diet supplemented with caffeine. Dams of group 3 were fed a 20% protein diet supplemented with caffeine and zinc. The amount of caffeine added to the maternal diet was 2 mg/100 g body weight; the amount of zinc was 0.6 g/kg of diet. At birth, pups were mixed within each group, and 8 randomly selected pups from each group were assigned to each dam of the respective group and were continuously fed the same diet. On day 15, the pups were killed and cranial bones, mandibles and femurs removed. The bones were measured, and the mineral content of the mandibles and femurs was determined. Although there were no differences in the dimensions of the cranial bones among the groups, the measurements and mineral content of the mandibles and femurs were consistently affected by the caffeine in the diet. On the other hand, supplementation of the caffeine-added diets with zinc led to greatly improved bone development, reaching values up to or beyond control levels. Thus zinc supplementation of a caffeine diet given to the dams during gestation and lactation can favourably influence the otherwise impaired bone development of their offspring.
在妊娠第9天，将怀孕的母鼠随机分为3组。第1组母鼠喂食20%蛋白质饮食作为对照。第2组母鼠喂食添加了咖啡因的20%蛋白质饮食。第3组母鼠喂食添加了咖啡因和锌的20%蛋白质饮食。添加到母鼠饮食中的咖啡因量为2毫克/100克体重；锌的量为0.6克/千克饮食。出生时，幼崽在每组内混合，从每组中随机选择8只幼崽分配给各自组的每只母鼠，并持续喂食相同的饮食。在第15天，处死幼崽并取出颅骨、下颌骨和股骨。测量骨骼，并测定下颌骨和股骨的矿物质含量。尽管各组颅骨尺寸没有差异，但下颌骨和股骨的测量值及矿物质含量持续受到饮食中咖啡因的影响。另一方面，在添加咖啡因的饮食中补充锌可使骨骼发育得到极大改善，达到或超过对照水平。因此，在妊娠和哺乳期给母鼠喂食添加咖啡因的饮食并补充锌，可对其后代原本受损的骨骼发育产生有利影响。
