Center for Rhizosphere Biology, Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO, 80523, USA.
United States Department of Agriculture-Agricultural Research Service, Soil-Plant-Nutrient Research Unit, Fort Collins, CO, 80526, USA.
New Phytol. 2013 Apr;198(1):264-273. doi: 10.1111/nph.12124. Epub 2013 Jan 25.
It is known that environmental factors can affect the biosynthesis of leaf metabolites. Similarly, specific pairwise plant-microbe interactions modulate the plant's metabolome by stimulating production of phytoalexins and other defense-related compounds. However, there is no information about how different soil microbiomes could affect the plant growth and the leaf metabolome. We analyzed experimentally how diverse soil microbiomes applied to the roots of Arabidopsis thaliana were able to modulate plant growth and the leaf metabolome, as assessed by GC-MS analyses. Further, we determined the effects of soil microbiome-driven changes in leaf metabolomics on the feeding behavior of Trichopulsia ni larvae. Soil microbiomes differentially impacted plant growth patterns as well as leaf metabolome composition. Similarly, most microbiome-treated plants showed inhibition to larvae feeding, compared with unamended control plants. Pyrosequencing analysis was conducted to determine the soil microbial composition and diversity of the soils used in this study. Correlation analyses were performed to determine relationships between various factors (soil microbial taxa, leaf chemical components, plant growth patterns and insect feeding behavior) and revealed that leaf amino acid content was positively correlated with both microbiome composition and insect feeding behavior.
已知环境因素会影响叶片代谢物的生物合成。同样，特定的植物-微生物相互作用会通过刺激植物抗毒素和其他防御相关化合物的产生来调节植物的代谢组。然而，目前尚不清楚不同的土壤微生物组如何影响植物的生长和叶片代谢组。我们通过 GC-MS 分析实验性地研究了施加到拟南芥根部的不同土壤微生物组如何能够调节植物的生长和叶片代谢组。此外，我们还确定了叶片代谢组学中由土壤微生物组驱动的变化对 Trichopulsia ni 幼虫取食行为的影响。土壤微生物组会对植物的生长模式以及叶片代谢组的组成产生不同的影响。同样，与未经修饰的对照植物相比，大多数经微生物组处理的植物对幼虫的取食表现出抑制作用。进行了焦磷酸测序分析以确定本研究中使用的土壤的微生物组成和多样性。进行了相关分析以确定各种因素（土壤微生物类群、叶片化学成分、植物生长模式和昆虫取食行为）之间的关系，并揭示了叶片氨基酸含量与微生物组组成和昆虫取食行为呈正相关。
