Department of Terrestrial Ecology, The Netherlands Institute of Ecology NIOO-KNAW, Droevendaalsesteeg 10, 6708 PB, Wageningen, The Netherlands.
Key Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetic and Developmental Biology, The Chinese Academy of Sciences, 286 Huaizhong Road, 050021, Shijiazhuang, Hebei, China.
Nat Commun. 2019 Mar 19;10(1):1254. doi: 10.1038/s41467-019-09284-w.
Microbiomes of soils and plants are linked, but how this affects microbiomes of aboveground herbivorous insects is unknown. We first generated plant-conditioned soils in field plots, then reared leaf-feeding caterpillars on dandelion grown in these soils, and then assessed whether the microbiomes of the caterpillars were attributed to the conditioned soil microbiomes or the dandelion microbiome. Microbiomes of caterpillars kept on intact plants differed from those of caterpillars fed detached leaves collected from plants growing in the same soil. Microbiomes of caterpillars reared on detached leaves were relatively simple and resembled leaf microbiomes, while those of caterpillars from intact plants were more diverse and resembled soil microbiomes. Plant-mediated changes in soil microbiomes were not reflected in the phytobiome but were detected in caterpillar microbiomes, however, only when kept on intact plants. Our results imply that insect microbiomes depend on soil microbiomes, and that effects of plants on soil microbiomes can be transmitted to aboveground insects feeding later on other plants.
土壤和植物的微生物组是相互关联的，但这如何影响地上食草昆虫的微生物组尚不清楚。我们首先在野外试验区生成了植物驯化的土壤，然后在这些土壤中种植蒲公英来饲养食叶毛毛虫，并评估毛毛虫的微生物组是归因于驯化的土壤微生物组还是蒲公英微生物组。保存在完整植株上的毛毛虫的微生物组与从生长在相同土壤中的植株上收集的离体叶片喂养的毛毛虫的微生物组不同。饲养在离体叶片上的毛毛虫的微生物组相对简单，类似于叶片微生物组，而来自完整植株的毛毛虫的微生物组则更加多样，类似于土壤微生物组。植物介导的土壤微生物组的变化并没有反映在植物微生物组中，而是在毛毛虫的微生物组中被检测到，但只有在保存在完整植株上时才会被检测到。我们的研究结果表明，昆虫微生物组依赖于土壤微生物组，而且植物对土壤微生物组的影响可以传递给以后取食其他植物的地上食草昆虫。
