Stephanou A, Jessop D S, Knight R A, Lightman S L
Medical Unit, Charing Cross and Westminster Medical School, London, England.
Brain Behav Immun. 1990 Mar;4(1):67-73. doi: 10.1016/0889-1591(90)90007-d.
Unstimulated normal leukocytes were studied for their expression of corticotrophin-releasing factor (CRF)-like mRNA and peptide. In situ hybridization demonstrated CRF mRNA in T and B lymphocytes and in neutrophils. Northern blot analysis also detected the presence of a CRF mRNA species of approximately 1.7 Kb from lymphocytes and a peptide that reacted with anti-CRF antiserum was also detected by radioimmunoassay. This lymphocyte-derived peptide eluted earlier than standard CRF on high performance liquid chromatography. These findings suggest that regulatory mechanisms within the hypothalamus and the immune system may utilize closely related peptides.
对未受刺激的正常白细胞进行了促肾上腺皮质激素释放因子（CRF）样mRNA和肽表达的研究。原位杂交显示T和B淋巴细胞以及中性粒细胞中有CRF mRNA。Northern印迹分析也检测到淋巴细胞中存在一种约1.7kb的CRF mRNA种类，并且放射免疫测定还检测到一种与抗CRF抗血清反应的肽。在高效液相色谱上，这种淋巴细胞衍生的肽比标准CRF洗脱得更早。这些发现表明下丘脑和免疫系统内的调节机制可能利用密切相关的肽。
