Imaki T, Nahon J L, Sawchenko P E, Vale W
Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, CA 92037.
Brain Res. 1989 Sep 4;496(1-2):35-44. doi: 10.1016/0006-8993(89)91050-0.
Immunohistochemical, in situ hybridization histochemical, and Northern blot methods were used to demonstrate and characterize the distribution of corticotropin-releasing factor immunoreactivity (CRF-IR) and mRNA in the rat olfactory system. Northern analysis demonstrated the presence of an mRNA species in the olfactory bulb indistinguishable from, and in greater abundance than, CRF mRNA isolated from whole hypothalamus. Results from hybridization histochemical and immunohistochemical studies converged to indicate that CRF is expressed in a majority of mitral and tufted cells in the main and accessory bulbs, and in subsets of granule and periglomerular cells. Consistent with cellular localizations in primary output neurons, a dense network of fine CRF-immunoreactive varicosities was demonstrated in the external plexiform layer of the olfactory bulb and in layer Ia of piriform cortex. Other acknowledged terminal fields of the projection neurons of the main and accessory bulbs also displayed CRF-IR. The results suggest that CRF is the most broadly distributed neuroactive agent yet charted in olfactory bulb somata. This peptide may serve as a modulator or co-transmitter of importance in several cell types in the main and accessory olfactory bulbs, including the principal output neurons.
采用免疫组织化学、原位杂交组织化学和Northern印迹法来证实和描述促肾上腺皮质激素释放因子免疫反应性（CRF-IR）及mRNA在大鼠嗅觉系统中的分布。Northern分析表明，嗅球中存在一种mRNA，与从整个下丘脑分离出的CRF mRNA难以区分，且丰度更高。杂交组织化学和免疫组织化学研究结果一致表明，CRF在主嗅球和副嗅球的大多数二尖瓣细胞和簇状细胞以及颗粒细胞和球周细胞亚群中表达。与初级输出神经元中的细胞定位一致，在嗅球的外丛状层和梨状皮质的Ia层中显示出密集的细CRF免疫反应性曲张网络。主嗅球和副嗅球投射神经元的其他公认终末区域也显示出CRF-IR。结果表明，CRF是迄今为止在嗅球躯体中分布最广泛的神经活性物质。这种肽可能作为一种调节剂或共递质，在主嗅球和副嗅球的几种细胞类型中具有重要作用，包括主要输出神经元。
