Yamaki Vitor Nagai, Gonçalves Thiago Barbosa, Coelho João Vitor Baia, Pontes Ruy Victor Simões, Costa Felipe Lobato da Silva, Brito Marcus Vinicius Henriques
Laboratory of Experimental Surgery, State University of Pará – Pará, Brazil.
Rev Col Bras Cir. 2012 Dec;39(6):529-33. doi: 10.1590/s0100-69912012000600014.
To evaluate the protective effect of remote ischemic per-conditioning in ischemia and reperfusion-induced renal injury.
Fifteen rats (Rattus norvegicus) were randomized into three groups (n = 5): Group Normality (GN), Control Ischemia and Reperfusion (GIR) and Group remote ischemic per-conditioning (GPER). With the exception of the GN group, all others underwent renal ischemia for 30 minutes. In group GPER we performed the ischemic remote per-conditioning, consisting of three cycles of ischemia and reperfusion applied every five minutes during the ischemic period, to the left hindlimb of the rats by means of a tourniquet. To quantify the lesions we measured serum levels of creatinine and urea, as well as analyzed renal histopathology.
The GPER group presented with better levels of urea (83.74 ± 14.58%) and creatinine (0.72 ± 26.14%) when compared to GIR group, approaching the GN group. Histopathologically, the lower levels of medullary congestion and hydropic degeneration were found in group GPER.
The remote ischemic per-conditioning had a significant protective effect on renal ischemia and reperfusion.
评估远程缺血预处理对缺血再灌注诱导的肾损伤的保护作用。
将15只大鼠（褐家鼠）随机分为三组（n = 5）：正常组（GN）、缺血再灌注对照组（GIR）和远程缺血预处理组（GPER）。除GN组外，其他组均经历30分钟的肾脏缺血。在GPER组中，我们通过止血带对大鼠左后肢进行缺血远程预处理，即在缺血期每隔5分钟进行三个缺血再灌注周期。为了量化损伤，我们测量了血清肌酐和尿素水平，并分析了肾脏组织病理学。
与GIR组相比，GPER组的尿素（83.74 ± 14.58%）和肌酐（0.72 ± 26.14%）水平更好，接近GN组。组织病理学上，GPER组的髓质充血和水样变性水平较低。
远程缺血预处理对肾脏缺血再灌注具有显著的保护作用。
