高渗盐溶液联合远程缺血预处理对大鼠肾脏缺血/再灌注损伤的影响。

Effects of hypertonic saline solution associated to remote ischemic perconditioning in kidney ischemia/reperfusion injury in rats.

作者信息

Brito Marcus Vinicius Henriques, Yasojima Edson Yuzur, Percário Sandro, Ribeiro Rubens Fernando Gonçalves, Cavalcante Lainy Carollyne da Costa, Monteiro Andrew Moraes, Couteiro Rodrigo Paracampo, Rodrigues Ivone Aline da Silva, Santos Hellen Aparecida Geyer Dos

机构信息

PhD, Full Professor, Department of Experimental Surgery, Universidade Estadual do Pará (UEPA), Belém-PA, Brazil. Conception, design and scientific content of the study, critical revision.

PhD, Full Professor, Department of Biological Sciences, UFPA, Belém-PA, Brazil. Conception, design and scientific content of the study, critical revision.

出版信息

Acta Cir Bras. 2017 Mar;32(3):211-218. doi: 10.1590/S0102-865020170030000005.

DOI:10.1590/S0102-865020170030000005

PMID:28403345

Abstract

PURPOSE

: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats.

METHODS

: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed.

RESULTS

: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage.

CONCLUSION

: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.

摘要

目的

评估高渗盐溶液联合远程缺血预处理对大鼠肾缺血/再灌注损伤的影响。

方法

25只雄性Wistar大鼠接受右肾切除术，并分为五组：假手术组（S）；肾缺血30分钟的缺血/再灌注组（I/R）；在肾脏缺血期间进行三个10分钟I/R周期的远程缺血预处理组（Per）；用高渗盐溶液（4ml/kg）治疗的高渗盐溶液组（HSS）；接受两种治疗的远程缺血预处理+高渗盐溶液组（Per+HSS）。再灌注后，采集血样分析血清尿素氮和肌酐水平。评估血浆和肾组织中的丙二醛（TBARS）以评估氧化应激。进行肾脏组织病理学检查。