Tufts University, Department of Biomedical Engineering, 4 Colby St. Medford, Massachusetts 02155, USA.
Macromol Biosci. 2013 Mar;13(3):311-20. doi: 10.1002/mabi.201200323. Epub 2013 Jan 24.
Sericin removal from the core fibroin protein of silkworm silk is a critical first step in the use of silk for biomaterial-related applications, but degumming can affect silk biomaterial properties, including molecular weight, viscosity, diffusivity and degradation behavior. Increasing the degumming time (10, 30, 60, and 90 min) decreases the average molecular weight of silk protein in solution, silk solution viscosity, and silk film glass-transition temperature, and increases the rate of degradation of a silk film by protease. Model compounds spanning a range of physical-chemical properties generally show an inverse relationship between degumming time and release rate through a varied degumming time silk coating. Degumming provides a useful control point to manipulate silk's material properties.
从家蚕丝的核心丝胶蛋白中去除丝胶是将丝用于生物材料相关应用的关键第一步，但脱胶会影响丝生物材料的特性，包括分子量、粘度、扩散率和降解行为。增加脱胶时间（10、30、60 和 90 分钟）会降低溶液中丝蛋白的平均分子量、丝溶液的粘度和丝膜的玻璃化转变温度，并增加蛋白酶降解丝膜的速率。跨越一系列物理化学性质的模型化合物通常通过不同脱胶时间的丝涂层表现出脱胶时间与释放率之间的反比关系。脱胶提供了一个有用的控制点，可以控制丝的材料特性。
