小鼠胚胎源性肿瘤的细胞遗传学分析。

Cytogenetic analysis of murine embryo-derived tumors.

作者信息

van Berlo R J, de Jong B, Oosterhuis J W, Dijkhuizen T, Buist J, Dam A

机构信息

Department of Pathology, University of Groningen, The Netherlands.

出版信息

Cancer Res. 1990 Jun 1;50(11):3416-21.

PMID:2334937

Abstract

The possible relationship among malignancy, differentiation, and chromosomal constitution of primary embryo-derived tumors was studied. Tumors were induced by transplanting 7-day-old mouse embryos under the kidney capsule of syngeneic BALB/c recipients. Transplantation of 101 embryos resulted in 18 tumor-bearing mice: 36 teratocarcinomas; 18 teratomas; and 27 yolk sac tumors. Some of the yolk sac tumors proved to be retransplantable for several generations. Cytogenetic investigation of the primary embryo-derived tumors revealed that the majority of teratocarcinomas (82%) were chromosomally normal, whereas almost all (83%) karyotyped teratomas and yolk sac tumors had a highly abnormal chromosomal constitution. Most common aberrations were polyploidy; overrepresentation of chromosome 1, 6, 15, or 19; and an underrepresentation of chromosome 2, 4, 14, or a sex chromosome.

摘要

研究了原发性胚胎源性肿瘤的恶性程度、分化程度与染色体构成之间的可能关系。通过将7日龄小鼠胚胎移植到同基因BALB/c受体的肾包膜下诱导肿瘤形成。移植101个胚胎后，有18只荷瘤小鼠：36个畸胎癌；18个畸胎瘤；27个卵黄囊瘤。部分卵黄囊瘤经证实可连续传代移植数代。对原发性胚胎源性肿瘤的细胞遗传学研究显示，大多数畸胎癌（82%）染色体正常，而几乎所有（83%）经核型分析的畸胎瘤和卵黄囊瘤染色体构成高度异常。最常见的畸变是多倍体；1号、6号、15号或19号染色体的过度表达；以及2号、4号、14号染色体或性染色体的表达不足。