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基于 AlgiMatrixTM 的 3D 细胞培养系统作为用于抗癌研究的体外肿瘤模型。

AlgiMatrix™ based 3D cell culture system as an in-vitro tumor model for anticancer studies.

机构信息

College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, United States of America.

出版信息

PLoS One. 2013;8(1):e53708. doi: 10.1371/journal.pone.0053708. Epub 2013 Jan 18.

DOI:10.1371/journal.pone.0053708
PMID:23349734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3548811/
Abstract

BACKGROUND

Three-dimensional (3D) in-vitro cultures are recognized for recapitulating the physiological microenvironment and exhibiting high concordance with in-vivo conditions. Taking the advantages of 3D culture, we have developed the in-vitro tumor model for anticancer drug screening.

METHODS

Cancer cells grown in 6 and 96 well AlgiMatrix™ scaffolds resulted in the formation of multicellular spheroids in the size range of 100-300 µm. Spheroids were grown in two weeks in cultures without compromising the growth characteristics. Different marketed anticancer drugs were screened by incubating them for 24 h at 7, 9 and 11 days in 3D cultures and cytotoxicity was measured by AlamarBlue® assay. Effectiveness of anticancer drug treatments were measured based on spheroid number and size distribution. Evaluation of apoptotic and anti-apoptotic markers was done by immunohistochemistry and RT-PCR. The 3D results were compared with the conventional 2D monolayer cultures. Cellular uptake studies for drug (Doxorubicin) and nanoparticle (NLC) were done using spheroids.

RESULTS

IC(50) values for anticancer drugs were significantly higher in AlgiMatrix™ systems compared to 2D culture models. The cleaved caspase-3 expression was significantly decreased (2.09 and 2.47 folds respectively for 5-Fluorouracil and Camptothecin) in H460 spheroid cultures compared to 2D culture system. The cytotoxicity, spheroid size distribution, immunohistochemistry, RT-PCR and nanoparticle penetration data suggested that in vitro tumor models show higher resistance to anticancer drugs and supporting the fact that 3D culture is a better model for the cytotoxic evaluation of anticancer drugs in vitro.

CONCLUSION

The results from our studies are useful to develop a high throughput in vitro tumor model to study the effect of various anticancer agents and various molecular pathways affected by the anticancer drugs and formulations.

摘要

背景

三维（3D）体外培养因其能够重现生理微环境并与体内条件高度一致而受到认可。利用 3D 培养的优势，我们已经开发出用于抗癌药物筛选的体外肿瘤模型。

方法

在 6 和 96 孔 AlgiMatrix™支架中生长的癌细胞在大小为 100-300 µm 的范围内形成多细胞球体。在不影响生长特性的情况下，将球体在培养物中培养两周。通过在 3D 培养物中孵育 24 小时，将不同的市售抗癌药物进行筛选，并通过 AlamarBlue®测定法测量细胞毒性。基于球体数量和大小分布来测量抗癌药物治疗的有效性。通过免疫组化和 RT-PCR 评估凋亡和抗凋亡标志物。将 3D 结果与传统的 2D 单层培养物进行比较。使用球体进行药物（阿霉素）和纳米颗粒（NLC）的细胞摄取研究。

结果

与 2D 培养模型相比，抗癌药物在 AlgiMatrix™系统中的 IC50 值显着更高。与 2D 培养系统相比，H460 球体培养物中 cleaved caspase-3 的表达显着降低（5-氟尿嘧啶和喜树碱分别降低 2.09 和 2.47 倍）。细胞毒性、球体大小分布、免疫组化、RT-PCR 和纳米颗粒渗透数据表明，体外肿瘤模型对抗癌药物的耐药性更高，支持 3D 培养是体外抗癌药物细胞毒性评估的更好模型的事实。

结论

我们的研究结果有助于开发高通量体外肿瘤模型，以研究各种抗癌药物和抗癌药物和制剂影响的各种分子途径的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/d311eb976cfa/pone.0053708.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/6487e2220372/pone.0053708.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/d311eb976cfa/pone.0053708.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/6487e2220372/pone.0053708.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/c5d4ea83ad99/pone.0053708.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/e1fe200132ad/pone.0053708.g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/6b12858fd44a/pone.0053708.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09c/3548811/d311eb976cfa/pone.0053708.g007.jpg

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本文引用的文献

1
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2
A novel in vitro three-dimensional retinoblastoma model for evaluating chemotherapeutic drugs.一种用于评估化疗药物的新型体外三维视网膜母细胞瘤模型。
Mol Vis. 2012;18:1361-78. Epub 2012 May 31.
3
β1 integrin: an emerging player in the modulation of tumorigenesis and response to therapy.β1 整合素：在肿瘤发生和治疗反应调节中的新兴参与者。
Clin Exp Metastasis. 2025 Jan 25;42(2):12. doi: 10.1007/s10585-025-10328-3.
4
Development of a hydrogel-based three-dimensional (3D) glioblastoma cell lines culture as a model system for CD73 inhibitor response study.开发一种基于水凝胶的三维（3D）胶质母细胞瘤细胞系培养体系，作为CD73抑制剂反应研究的模型系统。
Biomed Eng Online. 2024 Dec 21;23(1):127. doi: 10.1186/s12938-024-01320-1.
5
Full maturation of in vitro Plasmodium falciparum oocysts using the AlgiMatrix 3D culture system.利用 AlgiMatrix 3D 培养系统使体外疟原虫卵囊完全成熟。
Malar J. 2024 Aug 20;23(1):251. doi: 10.1186/s12936-024-05079-7.
6
Preclinical models for the study of pediatric solid tumors: focus on bone sarcomas.用于小儿实体瘤研究的临床前模型：聚焦骨肉瘤
Front Oncol. 2024 Jul 18;14:1388484. doi: 10.3389/fonc.2024.1388484. eCollection 2024.
7
Uniform sized cancer spheroids production using hydrogel-based droplet microfluidics: a review.使用基于水凝胶的液滴微流控技术制备均一尺寸的肿瘤球体：综述。
Biomed Microdevices. 2024 May 29;26(2):26. doi: 10.1007/s10544-024-00712-3.
8
Scaffold-based 3D cell culture models in cancer research.基于支架的 3D 细胞培养模型在癌症研究中的应用。
J Biomed Sci. 2024 Jan 14;31(1):7. doi: 10.1186/s12929-024-00994-y.
9
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10
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Cell Adh Migr. 2012 Mar-Apr;6(2):71-7. doi: 10.4161/cam.20077. Epub 2012 Mar 1.
4
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PLoS One. 2012;7(4):e34279. doi: 10.1371/journal.pone.0034279. Epub 2012 Apr 11.
5
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J Transl Med. 2012 Mar 6;10:38. doi: 10.1186/1479-5876-10-38.
6
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Biomaterials. 2012 Feb;33(5):1437-44. doi: 10.1016/j.biomaterials.2011.10.056. Epub 2011 Nov 10.
7
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9
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10
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