Pereira de Vasconcelos A, Colin C, Desor D, Divry M, Nehlig A
INSERM U272, Université de Nancy, France.
Exp Neurol. 1990 May;108(2):176-87. doi: 10.1016/0014-4886(90)90025-n.
The influence of an early chronic phenobarbital (PhB) exposure on local cerebral glucose utilization (LCGU) and on behavior was studied in the rat. The animals were treated from Postnatal Day 2 to Postnatal Day 35 by a daily injection of 50 mg/kg PhB or by saline and tested between 10 and 35 days for short-term effects of the drug on LCGU and between 70 and 90 days for long-term effects of PhB on LCGU and behavior. PhB induced short- and long-lasting reductions in the overall rates of LCGU in hippocampal and cerebellar areas, but no significant changes in LCGU in the different cell layers of these two cerebral areas. PhB also changed the pattern of maturation of the rates of LCGU as compared to control subjects. The barbiturate treatment induced a decrease in the exploratory behavior of PhB- as compared to saline-treated rats in the open field, as well as a significant 25% decrease in the rate of spontaneous alternation with delay. In addition, PhB-treated rats needed significantly more time than control animals to perform their trials in the nonrewarded T maze testing. However, the neonatal barbiturate exposure did not induce changes in performances of adult rats in the rewarded eight arm maze. The results of the present study show that there is no apparent correlation between the rates of energy metabolism in the hippocampus and the impairment of learning abilities of adult rats in behavioral tests related to the hippocampus.
研究了早期慢性苯巴比妥（PhB）暴露对大鼠局部脑葡萄糖利用（LCGU）和行为的影响。从出生后第2天至出生后第35天，对动物每日注射50 mg/kg PhB或生理盐水进行处理，并分别在10至35天测试药物对LCGU的短期影响，以及在70至90天测试PhB对LCGU和行为的长期影响。PhB导致海马体和小脑区域的LCGU总体速率出现短期和长期降低，但这两个脑区不同细胞层的LCGU没有显著变化。与对照对象相比，PhB还改变了LCGU速率的成熟模式。与生理盐水处理的大鼠相比，巴比妥酸盐处理导致PhB处理的大鼠在旷场中的探索行为减少，以及自发交替延迟率显著降低25%。此外，在无奖励的T迷宫测试中，PhB处理的大鼠比对照动物需要更多时间来完成试验。然而，新生儿期巴比妥酸盐暴露并未导致成年大鼠在奖励八臂迷宫中的表现发生变化。本研究结果表明，海马体中的能量代谢速率与成年大鼠在与海马体相关的行为测试中的学习能力受损之间没有明显相关性。
