Pereira de Vasconcelos A, Schroeder H, Nehlig A
INSERM U. 272, Université de Nancy, France.
Brain Res. 1987 Dec 1;433(2):231-6. doi: 10.1016/0165-3806(87)90026-5.
The influence of phenobarbital (PhB) on the utilization of beta-hydroxybutyrate by the cerebral cortex and the cerebellum was studied in rats during postnatal maturation. The animals were treated from day 2 to day 35 after birth either by a daily injection of 50 mg/kg PhB or by saline. The rats were studied at 5 postnatal stages: 7, 10, 14, 21 and 35 days. Plasma beta-hydroxybutyrate and acetoacetate levels reached their peak values between 10 and 14 days after birth. The concentration of both ketone bodies was significantly higher in PhB- than in saline-treated rats between 10 and 35 days after birth. The total incorporation of [3-14C]beta-hydroxybutyrate into amino acids reached a peak value at 14 days after birth and was down to very low values at 35 days. It was higher in PhB- than in saline-treated rats. The specific radioactivity values of glutamate, glutamine, aspartate and GABA were significantly higher in PhB- than in saline-treated especially at 10 days after birth. These results demonstrate that a PhB treatment induces an increase in brain ketone body utilization in neonate rats, which is likely to balance the decrease in brain glucose utilization induced by this pharmacological treatment.
在出生后成熟过程中，研究了苯巴比妥（PhB）对大鼠大脑皮层和小脑利用β-羟基丁酸酯的影响。从出生后第2天至第35天，每日给动物注射50 mg/kg PhB或生理盐水进行处理。在出生后的5个阶段对大鼠进行研究：7天、10天、14天、21天和35天。血浆β-羟基丁酸酯和乙酰乙酸水平在出生后10至14天达到峰值。在出生后10至35天之间，PhB处理组大鼠的两种酮体浓度均显著高于生理盐水处理组。[3-14C]β-羟基丁酸酯掺入氨基酸的总量在出生后14天达到峰值，在35天时降至非常低的值。PhB处理组大鼠的值高于生理盐水处理组。谷氨酸、谷氨酰胺、天冬氨酸和γ-氨基丁酸的比放射性值在PhB处理组中显著高于生理盐水处理组，尤其是在出生后10天。这些结果表明，PhB处理可诱导新生大鼠大脑酮体利用率增加，这可能平衡了这种药物处理引起的大脑葡萄糖利用率下降。
