Ege University, Bioengineering Department, EBILTEM Hall, 35040 Bornova-Izmir, Turkey.
Bioresour Technol. 2013 Mar;131:258-65. doi: 10.1016/j.biortech.2012.12.070. Epub 2012 Dec 20.
A green microalgae, named as Ettlia texensis was obtained from local freshwater in Turkey. The effects of autotrophic, photoheterotrophic and heterotrophic cultivations on biomass and lipid production were studied. Searching the preferences of the carbon and nitrogen source revealed that this strain could grow photoheterotrophically well with glucose and yeast extract. In the optimized medium, the highest biomass productivity and total lipid content achieved were 0.97 g/L d and 26% of dry weight basis, respectively. Moreover, the major fatty acid methyl esters were C16:0; C18:1; C18:2 and C18:3. In a scale-up attempt, productions were accomplished in a 3 L stirred tank bioreactor. The final biomass and lipid productivities obtained in bioreactor with 250 rpm agitation rate were 0.92 g/L d and 322 mg/L d, respectively. The biochemical compositions were monitored simultaneously by the FTIR spectroscopy during the production in bioreactor. E. texensis could be potent candidate for commercial production in the bioreactor photoheterotrophically.
从土耳其当地的淡水获得了一种名为 Ettlia texensis 的绿色微藻。研究了自养、光照异养和异养培养对生物量和脂质生产的影响。研究表明，该菌株可以很好地进行光照异养生长，利用葡萄糖和酵母提取物作为碳氮源。在优化的培养基中，最高的生物量生产力和总脂质含量分别达到 0.97 g/L/d 和 26%（干重基础）。此外，主要的脂肪酸甲酯为 C16:0；C18:1；C18:2 和 C18:3。在放大试验中，在 3 L 搅拌罐生物反应器中完成了生产。在搅拌速度为 250 rpm 的生物反应器中获得的最终生物量和脂质生产力分别为 0.92 g/L/d 和 322 mg/L/d。在生物反应器中生产过程中，通过傅里叶变换红外光谱法同时监测生物化学组成。E. texensis 可能是在生物反应器中进行光照异养生产的有潜力的商业生产候选者。
