Laboratory for Algae Research and Biotechnology, Department of Applied Sciences and Mathematics, Arizona State University Polytechnic Campus, Mesa, AZ 85212, United States.
Bioresour Technol. 2013 Feb;129:289-95. doi: 10.1016/j.biortech.2012.10.062. Epub 2012 Nov 5.
Microalgae have the ability to undergo programmatic changes in photosynthetic carbon partitioning and thus cellular biochemical composition, particularly in the relative amounts of crude protein, lipids, and carbohydrate, in response to changes in environmental and culture conditions. In this study, a novel strategy that employs a single microalgal strain Scenedesmus dimorphus grown in a single cultivation platform to produce protein-, carbohydrate- or lipid-rich biomass, as so desired, was introduced. With the combined manipulation of nitrogen availability and light intensity and cell inoculation density, it was successfully demonstrated that highest yields for protein and carbohydrate were 0.2 and 0.7 g L(-1) d(-1), respectively, which could be obtained in early stages of cultivation, while the highest yield for lipid, 0.17 g L(-1) d(-1), occured in a late stage of cultivation.
微藻具有在光合作用碳分配和细胞生化组成方面进行程序性变化的能力，特别是在粗蛋白、脂质和碳水化合物的相对含量方面，以响应环境和培养条件的变化。在这项研究中，引入了一种新的策略，即利用单一的微藻菌株 Scenedesmus dimorphus 在单一的培养平台中生长，以根据需要生产富含蛋白质、碳水化合物或脂质的生物质。通过联合操纵氮供应、光强度和细胞接种密度，成功地证明了在培养的早期阶段，蛋白质和碳水化合物的最高产量分别为 0.2 和 0.7 g L(-1) d(-1)，而脂质的最高产量为 0.17 g L(-1) d(-1)，则出现在培养的后期。
