Yokoi T, Sagisaka K
Department of Forensic Medicine, Tohoku University School of Medicine, Sendai, Japan.
Forensic Sci Int. 1990 Mar;45(1-2):39-46. doi: 10.1016/0379-0738(90)90219-o.
The stability of DNA in human bloodstains and various post mortem tissues has been investigated. High molecular weight (HMW) DNA was usually recovered from dried bloodstains, even those up to a few years old, but very rapid degradation was found to occur post mortem in the liver, pancreas, spleen and kidney. Other tissues such as the heart, thyroid and skeletal muscle were found to give a reasonable yield of HMW DNA during the first few days after death. The feasibility of using DNA extracted from forensic bloodstain specimens for the detection of DNA polymorphisms was explored using a human haptoglobin (Hp) alpha chain specific probe. Using HindIII and XbaI digests the Hp genotypes Hp2, Hp1F and Hp1S were distinguished by Southern blot analysis in DNA prepared from 1 cm2 bloodstains up to 15-18 months old.
人们对人血迹及各种死后组织中DNA的稳定性进行了研究。通常能从干血迹中提取出高分子量（HMW）DNA，即使是那些数年之久的血迹，但发现死后肝脏、胰腺、脾脏和肾脏中的DNA会迅速降解。发现心脏、甲状腺和骨骼肌等其他组织在死后头几天能产生相当数量的HMW DNA。使用人触珠蛋白（Hp）α链特异性探针，探讨了利用从法医血迹标本中提取的DNA检测DNA多态性的可行性。通过HindIII和XbaI酶切，采用Southern印迹分析法，在取自面积为1平方厘米、保存时间长达15 - 18个月的血迹所制备的DNA中区分出Hp基因型Hp2、Hp1F和Hp1S。
