通过聚丙烯酰胺凝胶等电聚焦进行触珠蛋白表型分析及其在血清蛋白同步分型中的应用。

Haptoglobin phenotyping by polyacrylamide gel isoelectric focusing and its application to simultaneous typing of serum proteins.

作者信息

Fukuda M, Tamaki Y, Kishida T

机构信息

Department of Forensic Medicine, Medical College of Oita, Japan.

出版信息

Z Rechtsmed. 1988;101(1):37-40. doi: 10.1007/BF00205322.

DOI:10.1007/BF00205322

PMID:3218386

Abstract

A simple isoelectric focusing method for haptoglobin (HP) typing is described. Serum was pretreated first with C. perfringens neuraminidase (CPN) and then with dithiothreitol (DTT). The treated serum was subjected to polyacrylamide gel isoelectric focusing (PAGIF), and the band patterns were detected by immunoblotting. The method could be successfully applied to HP typing of bloodstains as old as 2 months. A slight modification of it enabled HP, complement component C81, and factor I (IF) to be typed simultaneously. The immunoblotting facilitated preservation of HP patterns. Thus, the PAGIF method for HP typing is suitable for routine use in the forensic laboratory.

摘要

本文描述了一种用于触珠蛋白（HP）分型的简单等电聚焦方法。血清首先用产气荚膜梭菌神经氨酸酶（CPN）预处理，然后用二硫苏糖醇（DTT）处理。将处理后的血清进行聚丙烯酰胺凝胶等电聚焦（PAGIF），并通过免疫印迹检测条带模式。该方法可成功应用于长达2个月的陈旧血迹的HP分型。对其稍加修改就能同时对HP、补体成分C81和因子I（IF）进行分型。免疫印迹有助于保留HP模式。因此，用于HP分型的PAGIF方法适用于法医实验室的常规使用。