Yano A, Yamamoto Y, Miyaishi S, Ishizu H
Department of Legal Medicine, Okayama University Medical School, Japan.
Acta Med Okayama. 1998 Aug;52(4):173-81. doi: 10.18926/AMO/31301.
We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.
我们使用等位基因特异性引物对通过聚合酶链反应进行触珠蛋白（Hp）基因分型。用该方法鉴定出了Hp的六种主要基因型。在居住于爱媛县和冈山县的日本人中，等位基因频率估计为Hp2 = 0.723，Hp1s = 0.277。使用0.3 ng DNA和0.125微升血液即可进行Hp基因分型。对于在室温下放置一个月的全血以及在室温下放置三年的血迹也可行。在加热的血液样本中，在100℃加热2小时的样本中检测到了Hp2和Hp1s这两个等位基因。在血迹中，在100℃加热2小时和120℃加热30分钟的样本中检测到了Hp2和Hp1s。此外，在唾液、发根、组织切片和牙髓等非血液样本中也能检测到基因型。目前这种Hp基因分型方法有望成为法医分析中的一种有用方法。
