Department of Bioscience, University of Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom.
Fungal Genet Biol. 2013 Jun;55:54-66. doi: 10.1016/j.fgb.2013.01.001. Epub 2013 Jan 25.
Reproductive phase change from vegetative mycelium to the initiation of fruiting in Agaricus bisporus is regulated in large part by the sensing of environmental conditions. A model is proposed in which three separate environmental factors exert control at different stages of the reproductive developmental process change. The eight carbon volatile 1-octen-3-ol controls the early differentiation from vegetative hyphae to multicellular knots; temperature reduction is essential for the later differentiation of primodia; and carbon dioxide level exerts quantitative control on the number of fruiting bodies developed. Analysis of transcriptomic changes during the reproductive phase change was carried out with initiation-specific microarrays, and the newly published A. bisporus genome was used to analyse the promoter regions of differentially regulated genes. Our studies have shown there to be both early and late initiation responses relating to sensing of eight carbon volatiles and temperature respectively. A subset of 45 genes was transcriptionally regulated during the reproductive phase change which exhibited a range of functions including cell structure, nitrogen and carbon metabolism, and sensing and signalling. Three gene clusters linking increased transcription with developmental stage were identified. Analysis of promoter regions revealed cluster-specific conserved motifs indicative of co-ordinated regulation of transcription.
从营养菌丝体到双孢蘑菇生殖阶段的转变在很大程度上受环境条件感应的调控。提出了一个模型，其中三个独立的环境因素在生殖发育过程变化的不同阶段发挥控制作用。八碳挥发性 1-辛烯-3-醇控制着从营养菌丝体向多细胞结的早期分化；温度降低对于原基的后期分化是必不可少的；二氧化碳水平对发育的子实体数量进行定量控制。使用启动子特异性微阵列进行生殖阶段变化过程中的转录组分析，并使用新发布的双孢蘑菇基因组分析差异调节基因的启动子区域。我们的研究表明，存在与八碳挥发物和温度感应相关的早期和晚期启动反应。在生殖阶段变化过程中，有一组 45 个基因转录受到调控，表现出多种功能，包括细胞结构、氮和碳代谢以及感应和信号转导。鉴定出三个与转录增加相关的基因簇。启动子区域分析揭示了簇特异性保守基序，表明转录的协调调控。
