School of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Sci Rep. 2022 Jun 30;12(1):11075. doi: 10.1038/s41598-022-15382-5.
The genes associated with fruiting body formation of Sparasis latifolia are valuable for improving mushroom breeding. To investigate this process, 4.8 × 10 RNA-Seq reads were acquired from three stages: hyphal knot (SM), primordium (SP), and primordium differentiation (SPD). The de novo assembly generated a total of 48,549 unigenes, of which 71.53% (34,728) unigenes could be annotated by at least one of the KEGG (Kyoto Encyclopedia of Genes and Genomes), GO (Gene Ontology), and KOG (Eukaryotic Orthologous Group) databases. KEGG and KOG analyses respectively mapped 32,765 unigenes to 202 pathways and 19,408 unigenes to 25 categories. KEGG pathway enrichment analysis of DEGs (differentially expressed genes) indicated primordium initiation was significantly related to 66 pathways, such as "Ribosome", "metabolism of xenobiotics by cytochrome P450", and "glutathione metabolism" (among others). The MAPK and mTOR signal transduction pathways underwent significant adjustments during the SM to SP transition. Further, our research revealed the PI3K-Akt signaling pathway related to cell proliferation could play crucial functions during the development of SP and SPD. These findings provide crucial candidate genes and pathways related to primordium differentiation and development in S. latifolia, and advances our knowledge about mushroom morphogenesis.
与松乳菇子实体形成相关的基因可用于改良蘑菇育种。为了研究这一过程，我们从三个阶段（菌丝结（SM）、原基（SP）和原基分化（SPD））获得了 4.8×10 的 RNA-Seq 读段。从头组装共生成了 48549 个 unigenes，其中 71.53%（34728 个）unigenes至少可以被 KEGG（京都基因与基因组百科全书）、GO（基因本体论）和 KOG（真核同源群）数据库之一注释。KEGG 和 KOG 分析分别将 32765 个 unigenes映射到 202 条途径和 19408 个 unigenes映射到 25 个类别。差异表达基因（DEGs）的 KEGG 途径富集分析表明，原基起始与 66 条途径显著相关，如“核糖体”、“细胞色素 P450 介导的外源物质代谢”和“谷胱甘肽代谢”等。SM 到 SP 过渡期间，MAPK 和 mTOR 信号转导途径经历了显著的调整。此外，我们的研究还揭示了与细胞增殖相关的 PI3K-Akt 信号通路可能在 SP 和 SPD 的发育过程中发挥关键作用。这些发现为松乳菇原基分化和发育相关的关键候选基因和途径提供了依据，丰富了我们对蘑菇形态发生的认识。
