Institute for Research in Biomedicine (IRB-Barcelona), Baldiri Reixac 10, 08028 Barcelona, Spain.
Nat Cell Biol. 2013 Mar;15(3):241-8. doi: 10.1038/ncb2671. Epub 2013 Jan 27.
During interphase in Drosophila neuroblasts, the Centrobin (CNB)-positive daughter centriole retains pericentriolar material (PCM) and organizes an aster that is a key determinant of the orientation of cell division. Here we show that daughter centrioles depleted of CNB cannot fulfil this function whereas mother centrioles that carry ectopic CNB can. CNB co-precipitates with a set of centrosomal proteins that include γ-TUB, ANA2, CNN, SAS-4, ASL, DGRIP71, POLO and SAS-6. Following chemical inhibition of POLO or removal of three POLO phosphorylation sites present in CNB, the interphase microtubule aster is lost. These results demonstrate that centriolar CNB localization is both necessary and sufficient to enable centrioles to retain PCM and organize the interphase aster in Drosophila neuroblasts. They also reveal an interphase function for POLO in this process that seems to have co-opted part of the protein network involved in mitotic centrosome maturation.
在果蝇神经母细胞的间期，中心体蛋白（CNB）阳性的子中心体保留着中心粒周围物质（PCM），并组织了一个星状体，这是决定细胞分裂方向的关键因素。在这里，我们表明，缺乏 CNB 的子中心体不能履行这一功能，而携带异位 CNB 的母中心体可以。CNB 与一组中心体蛋白共沉淀，包括 γ-TUB、ANA2、CNN、SAS-4、ASL、DGRIP71、POLO 和 SAS-6。在化学抑制 POLO 或去除 CNB 中存在的三个 POLO 磷酸化位点后，间期微管星状体消失。这些结果表明，中心体 CNB 的定位对于使中心体保留 PCM 和在果蝇神经母细胞中组织间期星状体是必要的，也是充分的。它们还揭示了 POLO 在这个过程中的一个间期功能，它似乎已经采用了参与有丝分裂中心体成熟的蛋白质网络的一部分。
