Industrial Biotechnology Programme, Institute of Chemical and Engineering Sciences, Agency for Science, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island 627833, Singapore.
Appl Microbiol Biotechnol. 2013 May;97(10):4309-14. doi: 10.1007/s00253-013-4710-7. Epub 2013 Jan 25.
A thermophilic Bacillus coagulans WCP10-4 with tolerance to high concentration of glucose was isolated from soil and used to produce optically pure L-lactic acid from glucose and starch. In batch fermentation at pH 6.0, 240 g/L of glucose was completely consumed giving 210 g/L of L-lactic acid with a yield of 95 % and a productivity of 3.5 g/L/h. In simultaneous saccharification and fermentation at 50 °C without sterilizing the medium, 200 g/L of corn starch was completely consumed producing 202.0 g/L of L-lactic acid. To the best of our knowledge, this strain shows the highest osmotic tolerance to glucose among the strains ever reported for lactic acid production. This is the first report of simultaneous saccharification and fermentation of starch for lactic acid production under a non-sterilized condition.
从土壤中分离到一株嗜热凝结芽孢杆菌（Bacillus coagulans）WCP10-4，该菌能耐受高浓度葡萄糖，可用于从葡萄糖和淀粉生产光学纯 L-乳酸。在 pH 6.0 的分批发酵中，240 g/L 的葡萄糖完全消耗，生成 210 g/L 的 L-乳酸，产率为 95%，生产强度为 3.5 g/L/h。在不灭菌的条件下于 50°C 进行同步糖化发酵，200 g/L 的玉米淀粉完全消耗，生成 202.0 g/L 的 L-乳酸。据我们所知，该菌株对葡萄糖的渗透压耐受性是已报道的用于生产乳酸的菌株中最高的。这是首次在非灭菌条件下报道淀粉的同步糖化发酵生产乳酸。
