Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 21073, Hamburg, Germany.
Bioprocess Biosyst Eng. 2020 Jun;43(6):1027-1035. doi: 10.1007/s00449-020-02300-0. Epub 2020 Feb 13.
Propionic acid (PA) is a valuable organic acid for the food and feed industry, but no bioproduction at industrial scale exists so far. As product inhibition is a major burden for bioprocesses producing organic acids, in situ product removal (ISPR) is desirable. Here, we demonstrate a new strategy to produce PA with a co-culture coupled with ISPR using electrodialysis. Specifically, Bacillus coagulans first produces lactic acid (LA) from sugar(s) and LA is converted to PA using Veillonella criceti. Applying ISPR to the mentioned co-culture, the specific PA yield was increased from 0.35 to 0.39 g g compared to no ISPR usage. Furthermore, the productivity was increased from 0.63 to 0.7 g L h by applying ISPR. Additionally, it was shown that co-consumption of xylose and glucose led to a higher PA productivity of 0.73 g L h, although PA yield was only increased slightly up to 0.36 g g.
丙酸（PA）是食品和饲料工业中一种有价值的有机酸，但迄今为止，还没有在工业规模上进行生物生产。由于产物抑制是生物生产有机酸的主要负担，因此需要原位产物去除（ISPR）。在这里，我们展示了一种使用电渗析的新型策略，通过共培养物与 ISPR 结合来生产 PA。具体来说，凝结芽孢杆菌首先从糖(s)中产生乳酸（LA），然后使用韦荣球菌将 LA 转化为 PA。在提到的共培养物中应用 ISPR，与不使用 ISPR 相比，PA 的比产率从 0.35 增加到 0.39 g/g。此外，通过应用 ISPR，生产率从 0.63 增加到 0.7 g/L/h。此外，还表明共消耗木糖和葡萄糖会导致更高的 PA 生产率，为 0.73 g/L/h，尽管 PA 产率仅略有增加，达到 0.36 g/g。
