Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, Brussels, Belgium.
J Assist Reprod Genet. 2013 Mar;30(3):361-9. doi: 10.1007/s10815-013-9930-3. Epub 2013 Jan 25.
To compare two vitrification methods and two warming methods for human oocyte vitrification using a high security closed device in terms of survival, fertilization and embryo development.
For vitrification, oocytes were (1) immediately placed in equilibration solution or (2) they were gradually exposed to the cryoprotectants. For warming, oocytes were placed (1) in a 25 μl preheated (37 °C) thawing solution droplet that was put at room temperature for 1 min once the oocytes were inside or (2) in a 150 μl droplet for 1 minute at 37 °C.
Survival and preimplantation development were significantly lower when warming was performed in a small preheated droplet. There was no significant difference in survival and embryo development between the gradual or direct exposure to cryoprotectants.
Using this high security closed vitrification device a 90 % survival rate can be achieved when the oocytes are immediately warmed in a large volume at 37 °C.
使用高安全性封闭设备，比较两种玻璃化方法和两种升温方法对人卵母细胞玻璃化的影响，从存活率、受精和胚胎发育方面进行评估。
玻璃化时，卵母细胞（1）直接放入平衡液中，或（2）逐渐暴露于冷冻保护剂中。升温时，卵母细胞（1）放在预热（37°C）的 25μl 解冻液小液滴中，一旦卵母细胞进入小液滴，将小液滴放在室温下 1 分钟，或（2）在 37°C 的 150μl 液滴中放置 1 分钟。
在小预热液滴中进行升温时，存活率和胚胎前体发育显著降低。逐渐或直接暴露于冷冻保护剂中，存活率和胚胎发育无显著差异。
使用这种高安全性封闭的玻璃化设备，当卵母细胞在 37°C 的大体积中立即升温时，存活率可达到 90%。
