Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan.
Am J Trop Med Hyg. 2013 Apr;88(4):681-8. doi: 10.4269/ajtmh.12-0407. Epub 2013 Jan 28.
Japanese encephalitis virus (JEV) infection in mosquitoes was monitored in Vietnam from 2006 to 2008. A total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of JEV infection by assays for cytopathic effects in C6/36 cells and by RT-PCR to detect flavivirus RNA. Three JEV strains were isolated from Culex tritaeniorhynchus Giles collected in northern and southern Vietnam and two JEV strains were isolated from Culex vishnui Theobald collected in the highlands of Vietnam. Genetic and phylogenetic analyses, based on complete E gene nucleotide sequences, revealed that the five JEV strains were classified into the genotype I group and six amino acid differences were found in these five strains. These results indicated that multiple JEV genotype I populations are circulating countrywide in Vietnam, transmitted by bites of their Cx. tritaeniorhynchus and Cx. vishnui.
2006 年至 2008 年，越南对蚊子中的日本脑炎病毒（JEV）感染进行了监测。共收集了 15225 只蚊子，鉴定为五个属中的 26 个物种，并将 12621 只蚊子分为 447 个组，通过 C6/36 细胞的细胞病变效应检测和 RT-PCR 检测黄病毒 RNA 来检测 JEV 感染。从越南北部和南部收集的三带喙库蚊中分离出三株 JEV 株，从越南高地收集的淡色库蚊中分离出两株 JEV 株。基于完整 E 基因核苷酸序列的遗传和系统发育分析表明，这 5 株 JEV 株分为基因型 I 组，在这 5 株中发现了 6 个氨基酸差异。这些结果表明，多种 JEV 基因型 I 群体在越南全国范围内传播，由其携带的三带喙库蚊和淡色库蚊叮咬传播。
