Department of Chemistry, Brigham Young University-Idaho, Rexburg, ID 83460, USA.
Anal Bioanal Chem. 2013 Apr;405(10):3085-9. doi: 10.1007/s00216-013-6737-0. Epub 2013 Jan 29.
Single-dimension separations are routinely coupled in series to achieve two-dimensional separations, yet little has been done to simultaneously exploit multiple dimensions during separation. In this work, simultaneous chromatography and electrophoresis is introduced and evaluated for its potential to achieve two-dimensional separations. In simultaneous chromatography and electrophoresis, chromatography occurs via capillary action while an orthogonal electric field concurrently promotes electrophoresis in a second dimension. A novel apparatus with a dual solvent reservoir was designed to apply the concurrent electric field. Various compounds were used to characterize the apparatus and technique, i.e., vitamins, amino acids, and dyes. Improved separation is reported with equivalent analysis times in comparison to planar chromatography alone. The feasibility of simultaneously employing chromatography and electrophoresis in two dimensions is discussed.
一维分离通常串联连接以实现二维分离，但在分离过程中很少同时利用多个维度。在这项工作中，引入了同时色谱和电泳，并评估了其实现二维分离的潜力。在同时色谱和电泳中，色谱通过毛细作用发生，而正交电场同时在第二维中促进电泳。设计了一种具有双溶剂储液器的新型仪器来施加并发电场。使用各种化合物来表征仪器和技术，例如维生素、氨基酸和染料。与单独的平面色谱相比，报告了在等效分析时间内的改进分离。讨论了在二维中同时使用色谱和电泳的可行性。
