Mathijssen N M C, Sturm P D, Pilot P, Bloem R M, Buma P, Petit P L, Schreurs B W
Department of Orthopaedics, Reinier de Graaf Groep, Reinier de Graafweg 3/11, 2625 AD, Delft, The Netherlands,
Cell Tissue Bank. 2013 Dec;14(4):615-20. doi: 10.1007/s10561-013-9362-4. Epub 2013 Jan 29.
With bone impaction grafting, cancellous bone chips made from allograft femoral heads are impacted in a bone defect, which introduces an additional source of infection. The potential benefit of the use of pre-processed bone chips was investigated by comparing the bacterial contamination of bone chips prepared intraoperatively with the bacterial contamination of pre-processed bone chips at different stages in the surgical procedure. To investigate baseline contamination of the bone grafts, specimens were collected during 88 procedures before actual use or preparation of the bone chips: in 44 procedures intraoperatively prepared chips were used (Group A) and in the other 44 procedures pre-processed bone chips were used (Group B). In 64 of these procedures (32 using locally prepared bone chips and 32 using pre-processed bone chips) specimens were also collected later in the procedure to investigate contamination after use and preparation of the bone chips. In total, 8 procedures had one or more positive specimen(s) (12.5 %). Contamination rates were not significantly different between bone chips prepared at the operating theatre and pre-processed bone chips. In conclusion, there was no difference in bacterial contamination between bone chips prepared from whole femoral heads in the operating room and pre-processed bone chips, and therefore, both types of bone allografts are comparable with respect to risk of infection.
采用骨打压植骨技术时，将同种异体股骨头制成的松质骨碎片打压植入骨缺损处，这会引入额外的感染源。通过比较手术过程中不同阶段术中制备的骨碎片与预处理骨碎片的细菌污染情况，研究了使用预处理骨碎片的潜在益处。为了研究骨移植材料的基线污染情况，在实际使用或制备骨碎片之前的88例手术中采集了样本：44例手术使用术中制备的骨碎片（A组），另外44例手术使用预处理骨碎片（B组）。在其中64例手术（32例使用局部制备的骨碎片，32例使用预处理骨碎片）中，还在手术后期采集样本，以研究骨碎片使用和制备后的污染情况。总共8例手术有一个或多个阳性样本（12.5%）。手术室制备的骨碎片与预处理骨碎片的污染率无显著差异。总之，手术室用全股骨头制备的骨碎片与预处理骨碎片的细菌污染无差异，因此，两种类型的同种异体骨在感染风险方面具有可比性。
