Rodriguez-Barrueco Ruth, Marshall Netonia, Silva Jose M
Institute for Cancer Genetics, Columbia University, New York, NY, USA.
Methods Mol Biol. 2013;980:353-70. doi: 10.1007/978-1-62703-287-2_21.
RNA interference (RNAi) has emerged as a powerful genetic strategy to functionally interrogate the entire genome by loss-of-function studies. In the last years, several arrayed shRNA libraries that can silence almost all the human genome have been developed. The generation of new and more efficient shRNA plasmids has allowed performing genetic screens in a pooled fashion and provides the opportunity to investigate the entire genome finding relevant genes to any biological process. In this chapter, the pipeline and methods to perform a pooled shRNA screen are discussed.
RNA干扰（RNAi）已成为一种强大的遗传策略，可通过功能缺失研究对整个基因组进行功能探究。在过去几年中，已经开发出了几种能够沉默几乎所有人类基因组的阵列式短发夹RNA（shRNA）文库。新型且更高效的shRNA质粒的产生使得能够以汇集的方式进行遗传筛选，并有机会研究整个基因组，找到与任何生物学过程相关的基因。在本章中，将讨论进行汇集式shRNA筛选的流程和方法。
