Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
Acta Biochim Biophys Sin (Shanghai). 2012 Feb;44(2):103-12. doi: 10.1093/abbs/gmr116.
The discovery of RNA interference (RNAi) has revolutionized genetic analysis in mammalian cells. Loss-of-function RNAi screens enable rapid, functional annotation of the genome. Of the various RNAi approaches, pooled shRNA libraries have received considerable attention because of their versatility. A number of genome-wide shRNA libraries have been constructed against the human and mouse genomes, and these libraries can be readily applied to a variety of screens to interrogate the function of human and mouse genes in an unbiased fashion. We provide an introduction to the technical aspects of using pooled shRNA libraries for genetic screens.
RNA 干扰 (RNAi) 的发现彻底改变了哺乳动物细胞中的基因分析。基因功能丧失 RNAi 筛选能够快速、有效地注释基因组。在各种 RNAi 方法中，汇集的 shRNA 文库因其多功能性而受到广泛关注。已经构建了针对人类和小鼠基因组的许多全基因组 shRNA 文库，这些文库可以很容易地应用于各种筛选，以无偏倚的方式研究人类和小鼠基因的功能。我们提供了使用汇集 shRNA 文库进行遗传筛选的技术方面的介绍。
