Mousavi Seyedeh Fahime, Kheiri Masoumeh Tavassoti, Hosseini Seyed Masoud, Taghizadeh Mojgan, Fotouhi Fatemeh, Heydarchi Behnaz, Bashar Rouzbeh, Gomari Hosna
Department. of Microbiology, Faculty of Biological Sciences, Shahid-Beheshti University, Tehran. Iran. ; Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran.
Iran J Med Sci. 2011 Sep;36(3):172-7.
Influenza virus is a major infectious pathogen of the respiratory system causing a high degree of morbidity and mortality annually. The worldwide vaccines are decided and produced annually by World Health Organization and licensed companies based on the samples collected from all over the world. The aim of this study was to determine phylogenecity and heterogenecity of the circulating influenza isolates during 2008-2009 outbreaks in Tehran, compare them with the vaccine strains that were recommended by WHO for the same period.
Nasopharyngeal swabs (n=142) were collected from patients with influenza and influenza-like illness. Typing and subtyping of the isolates were performed using multiplex RT-PCR and phylogenetic analysis was carried out for hemagglutinin genes of the isolates.
Fifty out of 142 samples were positive for influenza A virus, and no influenza B virus was detected. Phylogenetic analyses revealed that the A/H1N1 isolates were related closely to A/Brisbane/59/2007, and the A/H3N2 isolates were close to A/Brisbane/10/2007 vaccine strains.
The findings of the present study demonstrate that the A/H1N1 was the predominant subtype of human influenza virus among the patients studied in Tehran during 2008-2009 winter seasons. In addition, some amino acid variation was found in Tehran/2008/H1N1 isolates from the 2008-2009 vaccine strain, but the H3N2 isolates showed higher genetic resemblance to the vaccine strain.
流感病毒是呼吸系统的主要传染性病原体，每年导致高度的发病率和死亡率。全球疫苗由世界卫生组织和持牌公司根据从世界各地收集的样本每年确定并生产。本研究的目的是确定2008 - 2009年德黑兰流感疫情期间流行的流感分离株的系统发育和异质性，并将它们与同期世界卫生组织推荐的疫苗株进行比较。
从流感患者和流感样疾病患者中采集鼻咽拭子（n = 142）。使用多重逆转录聚合酶链反应对分离株进行分型和亚型分析，并对分离株的血凝素基因进行系统发育分析。
142个样本中有50个甲型流感病毒呈阳性，未检测到乙型流感病毒。系统发育分析表明，A/H1N1分离株与A/布里斯班/59/2007密切相关，A/H3N2分离株与A/布里斯班/10/2007疫苗株接近。
本研究结果表明，A/H1N1是2008 - 2009年冬季德黑兰研究患者中人类流感病毒的主要亚型。此外，在2008 - 2009年疫苗株的德黑兰/2008/H1N1分离株中发现了一些氨基酸变异，但H3N2分离株与疫苗株显示出更高的基因相似性。
