Center for Biomedical Engineering, School of Engineering, Brown University, Providence, Rhode Island 02912, USA.
J Biomed Mater Res B Appl Biomater. 2013 Jul;101(5):677-88. doi: 10.1002/jbm.b.32870. Epub 2013 Jan 29.
Infection of titanium (Ti)-based orthopedic implants is a growing problem due to the ability of bacteria to develop a resistance to today's antibiotics. As an attempt to develop a new strategy to combat bacteria functions, Ti was anodized in the present study to possess different diameters of nanotubes. It is reported here for the first time that Ti anodized to possess 20 nm tubes then followed by heat treatment to remove fluorine deposited from the HF anodization electrolyte solution significantly reduced both S. aureus and S. epidermidis growth compared to unanodized Ti controls. It was further found that the sterilization method used for both anodized nanotubular Ti and conventional Ti played an important role in the degree of bacteria growth on these substrates. Overall, UV light and ethanol sterilized samples decreased bacteria growth, while autoclaving resulted in the highest amount of bacteria growth. In summary, this study indicated that through a simple and inexpensive process, Ti can be anodized to possess 20 nm tubes that no matter how sterilized (UV light, ethanol soaking, or autoclaving) reduces bacteria growth and, thus, shows great promise as an antibacterial implant material.
由于细菌具有抗药性，因此钛（Ti）基骨科植入物的感染是一个日益严重的问题。作为开发对抗细菌功能的新策略的尝试，本研究对 Ti 进行了阳极氧化处理，使其具有不同直径的纳米管。据报道，Ti 经阳极氧化处理后形成 20nm 纳米管，然后进行热处理以去除 HF 阳极氧化电解液中沉积的氟，与未经阳极氧化处理的 Ti 对照相比，这显著降低了金黄色葡萄球菌和表皮葡萄球菌的生长。进一步发现，用于阳极氧化纳米管 Ti 和传统 Ti 的灭菌方法在这些基底上细菌生长的程度中起着重要作用。总体而言，UV 光和乙醇消毒的样品减少了细菌的生长，而高压灭菌则导致了最多的细菌生长。总之，这项研究表明，通过简单且廉价的工艺，Ti 可以被阳极氧化以形成 20nm 纳米管，无论如何进行灭菌（UV 光、乙醇浸泡或高压灭菌），都可以减少细菌的生长，因此作为一种抗菌植入材料具有很大的应用前景。
