体内人卵巢癌中 P53 和 NFκBp65 表达的心理应激效应。
Dys-psychological Stress Effect on Expressions of P53 and NFκBp65 in Human Ovarian Carcinoma In Vivo.
机构信息
Department of Gynecology, Aviation General Hospital, Beijing 100012, China.
出版信息
Chin J Cancer Res. 2012 Sep;24(3):245-8. doi: 10.1007/s11670-012-0245-1.
OBJECTIVE
To investigate the dys-psychological stress effect on the growth of subcutaneous xenotransplanted tumor in nude mice bearing human epithelium ovarian carcinoma, and the influence on P53 and NFκBp65 expressions.
METHODS
The subcutaneous tumor xenografts were established by implanting human epithelium ovarian carcinoma tissues into nude mice and the dys-psychological stress model was established with restraint. The mice were randomized into the following four treatment groups with each group six mice respectively: tumor group (group A), normal saline intraperitoneal injection; tumor with stress group (group B), normal saline intraperitoneal injection; tumor therapy group (group C), cisplatin intraperitoneal injection; and tumor therapy with stress group (group D), cisplatin intraperitoneal injection. The expressions of P53 and NFκBp65 in tumor tissues were determined by Western blotting.
RESULTS
The expressions of P53 and NFκBp65 in each restraint group were enhanced compared with the control groups (P<0.05).
CONCLUSION
The dys-psychological stress may induce the high expressions of P53 and NFκBp65 proteins and further promote tumor growth.
目的
探讨心理应激对裸鼠人上皮性卵巢癌皮下移植瘤生长的影响及其对 P53 和 NFκBp65 表达的影响。
方法
将人上皮性卵巢癌组织植入裸鼠皮下建立皮下移植瘤模型，采用束缚法建立心理应激模型。将小鼠随机分为 4 组，每组 6 只：肿瘤组（A 组），腹腔注射生理盐水；肿瘤应激组（B 组），腹腔注射生理盐水；肿瘤治疗组（C 组），腹腔注射顺铂；肿瘤治疗应激组（D 组），腹腔注射顺铂。采用 Western blot 法检测肿瘤组织中 P53 和 NFκBp65 的表达。
结果
各束缚组 P53 和 NFκBp65 蛋白表达均高于对照组（P<0.05）。
结论
心理应激可能诱导 P53 和 NFκBp65 蛋白高表达，进而促进肿瘤生长。
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