Qingdao University, Qingdao, China.
Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Int J Med Sci. 2019 Oct 21;16(11):1510-1516. doi: 10.7150/ijms.33979. eCollection 2019.
: We aimed to explore small interfering (si)RNA silencing of ribonucleotide reductase M2 () gene combined with cisplatin for the treatment of human ovarian cancer in nude mice models of subcutaneous transplantation of tumor cells. : After conventional cultivation of human ovarian cancer cell line SKOV3 , SKOV3 cells were injected into the right back of nude mice by subcutaneous injection to establish the subcutaneous tumor models. Twenty-four tumor-burdened rats were randomly divided into four groups (n=6): siRNA group, siRNA in combination with cisplatin group, cisplatin group, and control group. Intraperitoneal injection of cisplatin and subcutaneous injection of siRNA were performed weekly. Tumor volume was measured, and tumor growth inhibition rate was calculated. expression at the mRNA and protein levels was detected by reverse transcription-polymerase chain reaction and immunohistochemistry. : In the siRNA group, the tumor volume and tumor growth inhibition rate were 249.60±20.46 mm³ and 36.39%, respectively. The tumor growth inhibition rate and tumor volume were significantly different between the siRNA and control groups (p<0.05). In the cisplatin group, the tumor volume and tumor growth inhibition rate were 249.86±12.46 mm³ and 41.10%, respectively. The tumor growth inhibition rate and tumor volume were significantly different between the cisplatin and control groups (p<0.05). In the siRNA + cisplatin group, the tumor volume reduced to 180.84±16.25 mm³ and the tumor growth inhibition rate was increased to 64.33%, which were significantly different compared with the control group (p<0.01). Significant downregulation of mRNA and protein expression in the tumor tissues was detected by reverse transcription polymerase chain reaction and immunohistochemistry assay (p<0.05). : siRNA alone or combined with cisplatin can effectively inhibit the growth of human ovarian cancer in nude mice models of subcutaneous transplantation of tumor cells. gene silencing may be a potential treatment regimen for ovarian cancer in future.
探索小干扰 RNA （siRNA）沉默核糖核苷酸还原酶 M2 （）基因联合顺铂治疗人卵巢癌细胞裸鼠皮下移植瘤模型。方法：常规培养人卵巢癌细胞系 SKOV3 后，采用皮下注射法将 SKOV3 细胞接种于裸鼠右背部建立皮下移植瘤模型。将 24 只荷瘤大鼠随机分为 4 组（n=6）：siRNA 组、siRNA 联合顺铂组、顺铂组和对照组。每周分别行顺铂腹腔注射和 siRNA 皮下注射。测量肿瘤体积并计算肿瘤生长抑制率。采用逆转录-聚合酶链反应和免疫组化法检测 mRNA 和蛋白水平的表达。结果：siRNA 组肿瘤体积和肿瘤生长抑制率分别为 249.60±20.46mm³和 36.39%。siRNA 组与对照组比较，肿瘤生长抑制率和肿瘤体积差异有统计学意义（p<0.05）。顺铂组肿瘤体积和肿瘤生长抑制率分别为 249.86±12.46mm³和 41.10%。顺铂组与对照组比较，肿瘤生长抑制率和肿瘤体积差异有统计学意义（p<0.05）。siRNA+顺铂组肿瘤体积缩小至 180.84±16.25mm³，肿瘤生长抑制率提高至 64.33%，与对照组比较差异有统计学意义（p<0.01）。逆转录-聚合酶链反应和免疫组化检测结果显示，肿瘤组织中 mRNA 和蛋白表达显著下调（p<0.05）。结论：siRNA 单独或联合顺铂能有效抑制人卵巢癌细胞裸鼠皮下移植瘤的生长。基因沉默可能是未来卵巢癌治疗的一种潜在方案。
