Khan Suphiya, Baunthiyal Mamta, Kumari Alka, Sharma Vinay
Deptt. of Bioscience and Biotechnology, Banasthali University, Banasthali Vidyapith--304 022, India.
J Environ Biol. 2012 Jul;33(4):745-50.
Syzygium cumini Linn. (Myrtaceae) is a medicinal tree (Jamun) used worldwide in treatment of diabetes. However, no molecular data is available on genetic polymorphism and its relationship, if any with fluoride pollution. In the present study, the genetic variability of two populations of S. cumini growing in fluoride rich soils and normal soils located in Rajasthan and Haryana regions of India, respectively was determined using random amplified polymorphic DNA (RAPD) markers. Different measures of diversity in Rajasthan populations: Shannon's index of phenotypic diversity (I) = 0.440; Nei's genetic diversity (h) = 0.292; effective number of alleles per locus (Ne) = 1.497; total species diversity (Hsp) = 0.307 and within population diversity (Hpop) = 0.158 showed high diversity in comparison to Haryana populations. Thus, it seems that Rajasthan population responds with increased genetic variation resulting possibly from new mutation that affect allele frequencies as a consequence of adaptation to contaminated environment. This may imply that the increased diversity levels may act as a buffer to combat fluoride stress. Cluster analysis and principal component analysis (PCA) results showed mixing between the populations.
蒲桃（使君子科）是一种药用树（乌墨），在全球范围内用于治疗糖尿病。然而，关于其遗传多态性及其与氟污染（若存在关系）的分子数据尚无可用信息。在本研究中，分别使用随机扩增多态性DNA（RAPD）标记，测定了印度拉贾斯坦邦和哈里亚纳邦地区生长在富氟土壤和正常土壤中的两个蒲桃种群的遗传变异性。拉贾斯坦邦种群的不同多样性度量：香农表型多样性指数（I）= 0.440；内氏遗传多样性（h）= 0.292；每个位点的有效等位基因数（Ne）= 1.497；总物种多样性（Hsp）= 0.307以及种群内多样性（Hpop）= 0.158，与哈里亚纳邦种群相比显示出较高的多样性。因此，似乎拉贾斯坦邦种群通过增加遗传变异做出反应，这可能是由于适应受污染环境导致影响等位基因频率的新突变所致。这可能意味着增加的多样性水平可作为对抗氟胁迫的缓冲。聚类分析和主成分分析（PCA）结果显示种群之间存在混合。
