利用点击共轭合成生物素化的环二鸟苷酸（c-di-gmp）和环二腺苷酸（c-di-amp）
Synthesis of biotinylated c-di-gmp and c-di-amp using click conjugation.
作者信息
Gaffney Barbara L, Stebbins Nicholas D, Jones Roger A
机构信息
Department of Chemistry and Chemical Biology Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2013;32(1):1-16. doi: 10.1080/15257770.2012.748196.
Abstract
The biotinylated c-di-GMP and c-di-AMP conjugates 10a/b were synthesized by a straightforward set of procedures from standard, commercially available phosphoramidites. Their availability should allow isolation and characterization of new protein and RNA receptors for these key bacterial signaling molecules.
摘要
生物素化的环二鸟苷酸（c-di-GMP）和环二腺苷酸（c-di-AMP）缀合物10a/b是通过一系列直接的程序，由标准的市售亚磷酰胺合成的。它们的可得性应有助于分离和表征这些关键细菌信号分子的新蛋白质和RNA受体。
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