Yan Xiang, Al-Hayek Samih, Huang Haifeng, Zhu Zhenshu, Zhu Wei, Guo Hongqian
Department of Urology, Affiliated Drum Tower Hospital, Nanjing University, Nanjing, PR China.
Scand J Urol. 2013 Apr;47(2):145-51. doi: 10.3109/00365599.2012.713000. Epub 2013 Jan 30.
The aim of this study was to assess the photodynamic effect of nanoparticles loaded with a photosensitizing nanomedicine, 5-aminolevulinic acid (5-ALA), on T24 bladder cancer cells in vitro.
The nanoprecipitation technique was successfully used to prepare the drug-loaded polymeric nanoparticles. The drug loading rate and the drug loading efficiency were determined by ultraviolet spectrophotometry. The size and morphology of nanoparticles were detected using dynamic light scattering (DLS) and transmission electron microscopy. Cytotoxicity to T24 bladder cancer cells was assessed by coincubating 5-ALA-loaded nanoparticles of different concentrations with T24 bladder cancer cells. The cell growth inhibitory rate was measured after irradiation by a 650 nm wavelength diode laser.
The drug loading rate of 5-ALA-loaded nanoparticles was 7% with a loading efficiency of 85%. The T24 cell growth inhibitory rates after incubation with 5.0, 10.0, 25.0 and 50.0 μg/ml 5-ALA-loaded nanoparticles were 73.19%, 79.95%, 83.86% and 89.74%, respectively, which demonstrated significantly higher cytotoxicity than those in the empty nanoparticle groups and 5-ALA free drug groups (p < 0.05).
5-ALA-loaded nanoparticles were successfully prepared and had a significantly enhanced photodynamic tumoricidal effect on bladder cancer cells in vitro.
本研究旨在评估负载有光敏纳米药物5-氨基酮戊酸（5-ALA）的纳米颗粒对体外培养的T24膀胱癌细胞的光动力效应。
采用纳米沉淀技术成功制备了载药聚合物纳米颗粒。通过紫外分光光度法测定载药率和载药效率。利用动态光散射（DLS）和透射电子显微镜检测纳米颗粒的大小和形态。将不同浓度的载5-ALA纳米颗粒与T24膀胱癌细胞共同孵育，评估其对T24膀胱癌细胞的细胞毒性。用波长650nm的二极管激光照射后，测量细胞生长抑制率。
载5-ALA纳米颗粒的载药率为7%，载药效率为85%。与5.0、10.0、25.0和50.0μg/ml载5-ALA纳米颗粒孵育后，T24细胞生长抑制率分别为73.19%、79.95%、83.86%和89.74%，其细胞毒性显著高于空纳米颗粒组和无5-ALA游离药物组（p<0.05）。
成功制备了载5-ALA纳米颗粒，其对体外膀胱癌细胞具有显著增强的光动力杀瘤效应。
