USDA-ARS U. S. Salinity Laboratory, Riverside, CA, USA.
FEMS Microbiol Ecol. 2013 Jun;84(3):542-54. doi: 10.1111/1574-6941.12083. Epub 2013 Feb 26.
Shiga toxin-producing Escherichia coli O157:H7 has been implicated in many foodborne illnesses. In this study, survival of E. coli O157:H7 in 32 soils from California (CA) and Arizona (AZ) was investigated. Our goal was to correlate the survival time of E. coli O157:H7 in soils with 16S rRNA pyrosequencing based bacterial community composition. Kohonen self-organizing map of survival and associated soil chemical, physical and biological variables using artificial neural network analysis showed that survival of E. coli O157:H7 in soils was negatively correlated with salinity (EC), but positively correlated with total nitrogen (TN) and water soluble organic carbon (WSOC). Bacterial diversity as determined by the Shannon diversity index had no significant (P = 0.635) effect on ttd, but individual bacterial phyla had different effects. The survival of E. coli O157:H7 was positively correlated with the abundances of Actinobacteria (P < 0.001) and Acidobacteria (P < 0.05), and negatively correlated with those of Proteobacteria and Bacteroidetes (P < 0.05). Our data showed that specific groups of bacteria correlate with the persistence of E. coli O157:H7 in soils thus opening new ways to study the influence of certain bacterial phyla on persistence of this pathogen and other related pathogens in complex environments.
产志贺毒素大肠杆菌 O157:H7 已被牵连到许多食源性疾病中。在这项研究中，我们调查了来自加利福尼亚州（CA）和亚利桑那州（AZ）的 32 种土壤中大肠杆菌 O157:H7 的存活情况。我们的目标是将大肠杆菌 O157:H7 在土壤中的存活时间与基于 16S rRNA 焦磷酸测序的细菌群落组成相关联。使用人工神经网络分析对生存情况以及相关土壤化学、物理和生物学变量进行的 Kohonen 自组织映射表明，大肠杆菌 O157:H7 在土壤中的存活与盐度（EC）呈负相关，但与总氮（TN）和水溶性有机碳（WSOC）呈正相关。通过香农多样性指数确定的细菌多样性对 ttd 没有显著影响（P=0.635），但个别细菌门有不同的影响。大肠杆菌 O157:H7 的存活与放线菌（P<0.001）和酸杆菌（P<0.05）的丰度呈正相关，与变形菌和拟杆菌的丰度呈负相关（P<0.05）。我们的数据表明，特定的细菌群体与大肠杆菌 O157:H7 在土壤中的持续存在相关，从而为研究某些细菌门对这种病原体和其他相关病原体在复杂环境中的持续存在的影响开辟了新的途径。
